Laughter Yoga × Reduced Salivary Cortisol · Evidence and Gaps Sheet

Required evidence, verification status and materials actually read
Item	AVerification status	BReading scope	CReview item	DCurrent findings	EWhy unverified	FRequired materials and conditions	GFurther research
1	Partly verified	Full text not readStored summary read	N01 · Evidence conditionWhat are the selected cortisol question and verdict?Required	The selection is reduced salivary cortisol. Original subclaim C and the explanation of a weak 46-level signal with insufficient laughter specificity and session-to-session consistency are retained. The parent is nonclinical stress C50. S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	The existing subclaim, grade and explanation versus the parent verdict	Material needed: The selection has no separate score, status or assessment axes. The explanatory number is not turned into a new structured score or filled with the parent score. This records needed materials; no new research was executed.
2	Partly verified	Full text not readStored summary read	N02 · Evidence conditionWho participated in the cortisol trials?Required	Ozturk included 75 first-year nursing students; Fujisawa included 120 healthy university students. The 70 psychiatric nurses belonged to a different stress trial. S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Trial-specific health, age, recruitment and baseline characteristics	Material needed: Exact eligibility, sex, age, medication and lifestyle factors and baseline values in the direct cortisol trials are unverified. This records needed materials; no new research was executed.
3	Partly verified	Full text not readStored summary read	N03 · Evidence conditionIs laughter itself the same as the combined program?Required	The original program combines laughter with clapping, breathing, movement and group participation. It is not treated as a laughter-only effect, and no new practice method is provided. S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Components involving laughter, clapping, breathing, movement and group participation	Material needed: Cortisol-trial-specific session components, instructors, contact and actual participation are unverified. This records needed materials; no new research was executed.
4	Partly verified	Full text not readStored summary read	N04 · Evidence conditionHow do acute, repeated and parent-program exposures differ?Required	Fujisawa used an acute 3-arm design; Ozturk used 8 sessions. The recent positive program of 8 sessions of 30 minutes in the parent explanation belongs to the Bilgihan stress-trial context. S01 S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Trial-specific actual exposure, schedules and observation times	Material needed: The parent-program specification is not applied to other cortisol trials. Exposure, adherence and observation windows in direct trials are unverified. This records needed materials; no new research was executed.
5	Partly verified	Full text not readStored summary read	N05 · Evidence conditionWhat comparison do humorous video and reading provide?Required	The trial in 120 healthy students had 3 arms: laughter yoga, humorous video and reading. Video results are not reassigned to laughter yoga. S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Control components, contact, emotion, breathing and direct contrasts	Material needed: Arm-specific expectations, contact, breathing and emotional components and exact between-arm contrasts are unverified. This records needed materials; no new research was executed.
6	Unverified	Full text not readStored summary read	N06 · Evidence conditionWhat were the repeated trial's controls, expectations and contact?Required	Ozturk is preserved as an 8-session randomized trial. The parent explanation describes insufficient active social controls as a limitation. S03	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Correspondence of controls, social contact and expectations	Material needed: The exact components, contact amount and expectation assessment of the Ozturk control are unverified. The parent's no-intervention label is not automatically assigned to this trial. This records needed materials; no new research was executed.
7	Partly verified	Full text not readStored summary read	N07 · Evidence conditionWere randomization and laboratory blinding verified?Required	The direct cortisol reports are randomized trials. Parent concerns about lack of blinding and combined instructor/assessor roles are distinguished as context from the recent stress trial. S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Allocation, concealment and collection, assay and analysis roles	Material needed: Trial-specific allocation procedures, assessment and laboratory blinding and responsible roles in the direct cortisol trials are unverified. This records needed materials; no new research was executed.
8	Partly verified	Full text not readStored summary read	N08 · Evidence conditionWhen were saliva samples repeatedly collected?Required	The original statements are retained: Ozturk was between-arm positive in only 3 of 8 sessions; Fujisawa showed acute cortisol change over time. S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Collection times, pre/post intervals, session windows and within-person repeats	Material needed: Collection times, pre/post-session intervals, repeat counts and daily conditions are unverified. These are not converted into daily averages or long-term reductions. This records needed materials; no new research was executed.
9	Unverified	Full text not readStored summary read	N09 · Evidence conditionWhat were the assay method and concentration units?Required	The endpoint of the direct reports is salivary cortisol. S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Assay, units, detection limits, batches and specimen quality	Material needed: Quantitative values, units, assay methods, detection limits, batches, specimen handling and quality criteria are unverified. This records needed materials; no new research was executed.
10	Unverified	Full text not readStored summary read	N10 · Evidence conditionCan baseline, final, change and between-arm effects be determined?Required	The stored summary contains mixed session results and changes over time; the original statement that exact between-arm confidence intervals are unverified is retained. S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Trial-, session- and arm-specific estimates and confidence intervals	Material needed: Arm-specific baseline and final values, changes, between-arm effects, confidence intervals and exact significance levels are unverified. No new effects or intervals are calculated. This records needed materials; no new research was executed.
11	Partly verified	Full text not readStored summary read	N11 · Evidence conditionDo positive results in some sessions establish a consistent overall reduction?Required	The original Ozturk result is between-arm positivity in only 3 of 8 sessions. S03	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Session-specific results, selection, multiplicity and prespecified contrasts	Material needed: Which sessions were positive, directions and magnitudes, exact results in other sessions, prespecification and multiplicity adjustment are unverified. This is not converted into consistent reduction across all sessions. This records needed materials; no new research was executed.
12	Partly verified	Full text not readStored summary read	N12 · Evidence conditionDoes acute change over time establish a laughter-yoga-specific effect?Required	The original Fujisawa statements of cortisol change over time and changes with humorous video are retained. S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Time effects, between-arm and group-by-time effects and prespecified comparisons	Material needed: Exact laughter-yoga between-arm and group-by-time contrasts, net effects, confidence intervals and baseline adjustment are unverified. Time change is not promoted to a laughter-specific effect. This records needed materials; no new research was executed.
13	Partly verified	Full text not readStored summary read	N13 · Evidence conditionAre DHEA and cortisol the same outcome?Required	The original Fujisawa result retains both cortisol change and null DHEA findings. S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Separate biomarker analyses, denominators and results	Material needed: The biomarkers are not combined or converted into general physiological improvement. Exact values and outcome hierarchy for each biomarker are unverified. This records needed materials; no new research was executed.
14	Partly verified	Full text not readStored summary read	N14 · Evidence conditionIs the total sample a valid saliva-analysis denominator?Required	Original total samples of 75 in Ozturk and 120 in Fujisawa are retained. S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Allocation, completion and arm- and session-specific valid specimens and missingness	Material needed: Arm-specific allocation, completion, saliva denominators, specimen exclusions and time-specific missingness are unverified. Complete validity and session-specific denominators are not created. This records needed materials; no new research was executed.
15	Unverified	Full text not readStored summary read	N15 · Evidence conditionHow were missing and repeated data analyzed?Required	The stored summary lacks details of quantitative analysis models and missing-data handling. S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Analysis populations, missing specimens, repeated-data models and adjustment	Material needed: Missing saliva, correlations across sessions, specimen exclusions and adjustment for baseline, time and lifestyle factors are unverified. This records needed materials; no new research was executed.
16	Partly verified	Full text not readStored summary read	N16 · Evidence conditionWere cortisol hierarchy and registration prespecified?Required	The original records are retained: Ozturk registration was unable to be verified, and Fujisawa has UMIN000019409. The UMIN identifier also appears in the original citation annotation. S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Primary/secondary outcomes, collection, analysis, registration and amendments	Material needed: Registration originals and times, cortisol hierarchy, protocols, analysis plans and amendments are unread. An identifier alone does not certify prespecification. This records needed materials; no new research was executed.
17	Partly verified	Full text not readStored summary read	N17 · Evidence conditionDoes a large PSS statistic establish a cortisol effect?Required	Bilgihan's 70 participants, all completing at 35 versus 35, and PSS F(1,68)=251.56, P<.001, partial eta-squared=.787 are original parent stress-trial values. S01	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Scope and role of the parent stress outcome	Material needed: These statistics are not used as salivary cortisol effects or denominators or as disorder-treatment evidence. The original report's endpoint list does not include cortisol. This records needed materials; no new research was executed.
18	Partly verified	Full text not readStored summary read	N18 · Evidence conditionAre conflicting abstract and main-text statements cortisol results?Required	The original Yaş account retains 83 analyzed, 41 versus 42, coexisting abstract null and main-text multivariate interaction F=10.69, P<.001, and retrospective registration. S02	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Attribution of statements and multivariate tests to their own endpoints	Material needed: These statistics and conflicts are not reassigned as direct cortisol effects or exact null effects. Original endpoints are well-being, PSS and self-efficacy. This records needed materials; no new research was executed.
19	Unverified	Full text not readStored summary read	N19 · Evidence conditionDoes a short-term biomarker change establish clinical meaning and persistence?Required	The original explanation retains mixed cortisol results and gaps in exact between-arm confidence intervals as a separate weak signal. S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Meaning of change, long-term outcomes and disorder outcomes	Material needed: Direct links to normalization, meaningful change, long-term persistence and depression/anxiety treatment are unverified. This records needed materials; no new research was executed.
20	Unverified	Full text not readStored summary read	N20 · Evidence conditionWas independent replication under the same conditions established?Required	Single-exposure and 8-session direct reports are distinguished from separate stress reports. Group participation, breathing, attention and comedy effects are not combined as laughter-specific effects. S01 S02 S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	The same biomarker, comparator, duration and report/cohort relationships	Material needed: Independent confirmation under the same comparison conditions, heterogeneity and unique trial and participant totals are unverified. This records needed materials; no new research was executed.
21	Unverified	Full text not readStored summary read	N21 · Evidence conditionWhat were concurrent factors and collection environments?Supplementary	The original explanation distinguishes the combined program from social-participation and breathing effects. S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Research records of treatment, lifestyle, performance and collection conditions	Material needed: Medication, lifestyle, activities, collection environments and adherence in direct cortisol trials are unverified. Causes of biomarker changes are not newly established. This records needed materials; no new research was executed.
22	Unverified	Full text not readStored summary read	N22 · Evidence conditionWhat support and conflicts were disclosed in direct cortisol reports?Required	The original record that funding in Ozturk and Fujisawa was unable to be verified is retained. S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Funding, training support, employee authors and conflicts	Material needed: Evidence is lacking to establish the absence of support, affiliations, conflicts or involvement, or to certify independence. This records needed materials; no new research was executed.
23	Partly verified	Full text not readStored summary read	N23 · Evidence conditionDoes a no-support declaration verify role separation and independence?Required	Bilgihan's declaration of no external funding or conflicts is retained alongside combined instructor/assessor roles. S01	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Support/conflict declarations and instructor/assessor roles	Material needed: The declaration is not converted into confirmation of all support, blinding or independence. Original support disclosures were not read in this work. This records needed materials; no new research was executed.
24	Partly verified	Full text not readStored summary read	N24 · Evidence conditionWhat context do other stress trials' registration and support provide?Required	Yaş's unverified support and retrospective NCT06175936 registration and Bilgihan's NCT07015372 date-consistency concerns are retained separately. S01 S02	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Unverified support, retrospective registration and the scope of originals	Material needed: The registration, support and amendment originals are unread. They do not substitute for prespecification in direct cortisol trials. This records needed materials; no new research was executed.
25	Unverified	Full text not readStored summary read	N25 · Evidence conditionHow were harms collected and compared?Supplementary	Parent safety is Unknown, with insufficient arm-specific adverse-event and long-term safety numbers. A biomarker reduction is not converted into safety. S01 S02 S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Harms definitions, events, denominators, exposure, discontinuation and missingness	Material needed: Harms events, denominators, arm-specific comparisons, timing, missingness and discontinuation data are unverified. This records needed materials; no new research was executed.
26	Unverified	Full text not readStored summary read	N26 · Evidence conditionWhat are the boundaries for long-term and vulnerable-population harms and guidance?Supplementary	The parent safety explanation is preserved as historical context. No new yoga or mental-health treatment instructions are created. S01 S02 S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Populations, concurrent exposures, long-term harms and the original safety explanation	Material needed: Long-term and vulnerable-population evidence, alternative causes and concurrent exposures are unverified. Unknown is not converted into safe or no events. This records needed materials; no new research was executed.
27	Partly verified	Full text not readStored summary read	N27 · Evidence conditionHow are explanatory numbers, languages and parent axes retained?Required	Parent C50, Korean/English originals, unspecified precision, evidence date 2026-09-07, an empty corrections list and empty deliberation are retained. The subclaim C's 46-level and English C46 expressions are original explanation, with no separate score. S01 S02 S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Original Korean/English explanations, parent precision and corrections	Material needed: Historical review and explanatory numbers are not inherited as new structured scores, current review or certification. This records needed materials; no new research was executed.
28	Partly verified	Full text not readStored summary read	N28 · Evidence conditionWhat was actually read, and what is the status of external materials?Required	The complete stored parent summary and bibliography were read for production. Material links, reading of stored summaries and unread external materials are distinguished. S01 S02 S03 S04	Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.	Reading status of stored summaries, originals, registration, plans and support	Material needed: External papers, registration, protocols, analysis plans and support disclosures were unread and access was not attempted. Historical verification and URLs are not promoted to current reading. This records needed materials; no new research was executed.

No items match your search or filter.

28 itemsWhat the statuses mean

Assessment scope

Stored-material review date: Not recorded
Original evidence cut-off: 2026-09-07
Scope actually checked: Existing review summaries and stored records of methodological application. Full papers, supplements, registrations, plans and original data were not read.
What the dates mean: This is the date the stored materials were checked, not the date of a new search of the clinical literature.
Population: Direct cortisol reports involve 75 first-year nursing students and 120 healthy university students. Parent stress reports involving 70 psychiatric nurses and 83 nursing students provide separate context. Psychiatric nurses are an occupational sample, not diagnosed patients. Direct-trial eligibility, baseline, medication and lifestyle factors and applicability to other populations are unverified.
Ingredient, formulation and route: The program combines laughter, clapping, breathing, movement and group participation. Humorous video is a separate intervention. Components, instructors, contact, expectations, adherence and laughter-only contributions in direct cortisol trials are unverified. Recorded research exposure is not converted into new yoga, breathing or mental-health treatment instructions.
Dose: Ozturk's 8 sessions and Fujisawa's acute single exposure are separate conditions. The parent program of 8 sessions of 30 minutes belongs to the Bilgihan stress-trial context. That specification is not transferred to direct cortisol trials. Actual participation, adherence and correspondence between saliva-collection times and exposure are unverified for each direct trial.
Comparator: Fujisawa has 3 arms: laughter yoga, humorous video and reading. Ozturk's exact control, contact and expectations are unverified. Parent stress-trial no-intervention controls and combined roles are not automatically assigned to direct cortisol trials. Video-related and time-related changes are not converted into laughter-yoga-specific between-arm effects.
Measured outcome: The selection is reduced salivary cortisol. Distinguish Ozturk's between-arm positivity in 3 of 8 sessions from Fujisawa's time change, changes with video and null DHEA result. Original subclaim C and the explanatory 46-level wording are retained without a separate score. Exact units, collection, direct between-arm effects, confidence intervals, denominators, models, hierarchy and clinical meaning are unverified.
Duration: Acute single-exposure and 8-session results are kept separate. Session-specific collection intervals, daily conditions, persistence after completion and long-term observation are unverified. Short-term salivary cortisol changes are not extended to daily averages, long-term normalization, improved perceived stress or treatment of diagnosed patients.
Questions outside this scope: Positive findings in some sessions are not converted into consistent reduction across all sessions, new rates or pooled effects.; Time change and video-related change are distinguished from direct laughter-yoga-specific between-arm effects.; Cortisol, DHEA, PSS, GHQ, burnout, well-being, self-efficacy and mental symptoms are distinct outcomes.; Large parent PSS/multivariate statistics and conflicting statements are not reassigned as direct cortisol values or exact null effects.; Parent samples, exposure, controls, blinding and roles are not automatically assigned to direct cortisol trials.; Total samples are not used as valid specimen denominators for each arm/session, and no new unique participant, trial or event totals are created.; Original explanatory numbers are not turned into structured scores, and biomarker changes are not converted into safety, long-term normalization or treatment of diagnosed conditions.; No new yoga, breathing or mental-health treatment methods are provided.

Evidence and sources

The links below lead to the sources. A link alone does not mean the original source was read or its latest publication status verified.

S01 · Bilgihan S, Dost A. The effect of laughter yoga on the general health status, perceived stress and burnout levels of psychiatric nurses: a randomized controlled study. Discover Psychology. 2026;6:200. DOI: 10.1007/s44202-026-00725-4.

Official source

Material type: Randomized controlled trial
Materials actually read: Stored summary read; external materials unread
Findings recorded in the stored summary: PSS group-by-time F(1,68)=251.56, P<.001, partial eta-squared .787; trial and registration dates raised timing concerns
Record level: Parent stress-trial PSS/GHQ/burnout context: 70 participants, all completing at 35 versus 35. The original list has no salivary cortisol endpoint; these statistics, denominators, program and combined roles are not reassigned as direct biomarker results.
Study overlap and independent replication: The parent, perceived-stress and cortisol subclaims share the four original reports. Direct cortisol reports are distinguished from background stress reports. Original report-specific groups are retained, but exact recruitment, cohort and registration relationships and unique trial, participant and event counts are unverified. Other sheets, arms and sessions are not added as new independent replications.

Public availability, access and reading status by material
Material	Public availability	Access status	Actually read	Notes
Stored existing review summary	Preserved copy of the complete parent-verdict stored summary	Stored summary	Summary read	Preserve original DOI 10.1007/s44202-026-00725-4, NCT07015372 and timing concerns. PMID/PMCID are unrecorded. No-external-funding/COI declarations and combined instructor/assessor roles provide parent stress context, not current full-text or registration-content verification.
External paper	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original DOI 10.1007/s44202-026-00725-4, NCT07015372 and timing concerns. PMID/PMCID are unrecorded. No-external-funding/COI declarations and combined instructor/assessor roles provide parent stress context, not current full-text or registration-content verification.
Registration material	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original DOI 10.1007/s44202-026-00725-4, NCT07015372 and timing concerns. PMID/PMCID are unrecorded. No-external-funding/COI declarations and combined instructor/assessor roles provide parent stress context, not current full-text or registration-content verification.
Protocol	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original DOI 10.1007/s44202-026-00725-4, NCT07015372 and timing concerns. PMID/PMCID are unrecorded. No-external-funding/COI declarations and combined instructor/assessor roles provide parent stress context, not current full-text or registration-content verification.
Statistical analysis plan	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original DOI 10.1007/s44202-026-00725-4, NCT07015372 and timing concerns. PMID/PMCID are unrecorded. No-external-funding/COI declarations and combined instructor/assessor roles provide parent stress context, not current full-text or registration-content verification.
Funding, product-support and COI material	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original DOI 10.1007/s44202-026-00725-4, NCT07015372 and timing concerns. PMID/PMCID are unrecorded. No-external-funding/COI declarations and combined instructor/assessor roles provide parent stress context, not current full-text or registration-content verification.

S02 · Yaş MA, et al. The effect of laughter yoga on well-being, perceived stress, and academic self-efficacy in nursing students: A randomized controlled trial. Appl Psychol Health Well Being. 2025;17:e12610. PMID: 39415513. PMCID: PMC11635907. DOI: 10.1111/aphw.12610.

PubMed

Material type: Randomized controlled trial
Materials actually read: Stored summary read; external materials unread
Findings recorded in the stored summary: A null between-group abstract statement coexisted with a multivariate interaction F=10.69, P<.001; registration was retrospective
Record level: Parent stress context: 83 analyzed, 41 versus 42. Original abstract between-arm null, main-text multivariate interaction and retrospective-registration statements are retained together. Well-being, PSS and self-efficacy statistics are not converted into cortisol effects or exact null effects.
Study overlap and independent replication: The parent, perceived-stress and cortisol subclaims share the four original reports. Direct cortisol reports are distinguished from background stress reports. Original report-specific groups are retained, but exact recruitment, cohort and registration relationships and unique trial, participant and event counts are unverified. Other sheets, arms and sessions are not added as new independent replications.

Public availability, access and reading status by material
Material	Public availability	Access status	Actually read	Notes
Stored existing review summary	Preserved copy of the complete parent-verdict stored summary	Stored summary	Summary read	Preserve original PMID 39415513, PMC11635907, DOI 10.1111/aphw.12610 and NCT06175936. Abstract/main-text conflict and retrospective registration are original narratives, not newly resolved. Funding is unverified; these are not direct salivary cortisol results.
External paper	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 39415513, PMC11635907, DOI 10.1111/aphw.12610 and NCT06175936. Abstract/main-text conflict and retrospective registration are original narratives, not newly resolved. Funding is unverified; these are not direct salivary cortisol results.
Registration material	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 39415513, PMC11635907, DOI 10.1111/aphw.12610 and NCT06175936. Abstract/main-text conflict and retrospective registration are original narratives, not newly resolved. Funding is unverified; these are not direct salivary cortisol results.
Protocol	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 39415513, PMC11635907, DOI 10.1111/aphw.12610 and NCT06175936. Abstract/main-text conflict and retrospective registration are original narratives, not newly resolved. Funding is unverified; these are not direct salivary cortisol results.
Statistical analysis plan	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 39415513, PMC11635907, DOI 10.1111/aphw.12610 and NCT06175936. Abstract/main-text conflict and retrospective registration are original narratives, not newly resolved. Funding is unverified; these are not direct salivary cortisol results.
Funding, product-support and COI material	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 39415513, PMC11635907, DOI 10.1111/aphw.12610 and NCT06175936. Abstract/main-text conflict and retrospective registration are original narratives, not newly resolved. Funding is unverified; these are not direct salivary cortisol results.

S03 · Ozturk FO, Tezel A. Effect of laughter yoga on mental symptoms and salivary cortisol levels in first-year nursing students: A randomized controlled trial. Int J Nurs Pract. 2021;27:e12924. PMID: 33580606. DOI: 10.1111/ijn.12924.

PubMed

Material type: Eight-session randomized controlled trial
Materials actually read: Stored summary read; external materials unread
Findings recorded in the stored summary: Mental symptoms favored intervention; cortisol was between-group positive in only 3 of 8 sessions
Record level: Directly relevant original account: 75 participants over 8 sessions, with between-arm cortisol positivity in 3 of 8 sessions. Positive mental-symptom findings are separate. Session- and arm-specific concentrations, specimen denominators, direct contrasts, CIs, collection, assays, prespecified hierarchy and multiplicity are unverified.
Study overlap and independent replication: The parent, perceived-stress and cortisol subclaims share the four original reports. Direct cortisol reports are distinguished from background stress reports. Original report-specific groups are retained, but exact recruitment, cohort and registration relationships and unique trial, participant and event counts are unverified. Other sheets, arms and sessions are not added as new independent replications.

Public availability, access and reading status by material
Material	Public availability	Access status	Actually read	Notes
Stored existing review summary	Preserved copy of the complete parent-verdict stored summary	Stored summary	Summary read	Preserve original PMID 33580606 and DOI 10.1111/ijn.12924. PMCID is unrecorded; registration and support are unverified. Between-arm positivity in some sessions is not extended to consistent overall reduction; external originals and plans were unread in this work.
External paper	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 33580606 and DOI 10.1111/ijn.12924. PMCID is unrecorded; registration and support are unverified. Between-arm positivity in some sessions is not extended to consistent overall reduction; external originals and plans were unread in this work.
Registration material	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 33580606 and DOI 10.1111/ijn.12924. PMCID is unrecorded; registration and support are unverified. Between-arm positivity in some sessions is not extended to consistent overall reduction; external originals and plans were unread in this work.
Protocol	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 33580606 and DOI 10.1111/ijn.12924. PMCID is unrecorded; registration and support are unverified. Between-arm positivity in some sessions is not extended to consistent overall reduction; external originals and plans were unread in this work.
Statistical analysis plan	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 33580606 and DOI 10.1111/ijn.12924. PMCID is unrecorded; registration and support are unverified. Between-arm positivity in some sessions is not extended to consistent overall reduction; external originals and plans were unread in this work.
Funding, product-support and COI material	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 33580606 and DOI 10.1111/ijn.12924. PMCID is unrecorded; registration and support are unverified. Between-arm positivity in some sessions is not extended to consistent overall reduction; external originals and plans were unread in this work.

S04 · Fujisawa A, et al. Effect of laughter yoga on salivary cortisol and dehydroepiandrosterone among healthy university students: A randomized controlled trial. Complement Ther Clin Pract. 2018;32:6-11. PMID: 30057059. DOI: 10.1016/j.ctcp.2018.04.005.

PubMed

Material type: Three-arm laughter-yoga, humorous-video, and reading randomized trial
Materials actually read: Stored summary read; external materials unread
Findings recorded in the stored summary: Cortisol changed over time, humorous video also changed it, and DHEA was null
Record level: Directly relevant original account from an acute 3-arm comparison in 120 healthy students: cortisol changed over time, video also changed and DHEA was null. Exact laughter-yoga between-arm and group-by-time contrasts, CIs, denominators, collection and long-term persistence are unverified.
Study overlap and independent replication: The parent, perceived-stress and cortisol subclaims share the four original reports. Direct cortisol reports are distinguished from background stress reports. Original report-specific groups are retained, but exact recruitment, cohort and registration relationships and unique trial, participant and event counts are unverified. Other sheets, arms and sessions are not added as new independent replications.

Public availability, access and reading status by material
Material	Public availability	Access status	Actually read	Notes
Stored existing review summary	Preserved copy of the complete parent-verdict stored summary	Stored summary	Summary read	Preserve original PMID 30057059, DOI 10.1016/j.ctcp.2018.04.005 and UMIN000019409. PMCID is unrecorded; support and exact comparative CIs are unverified. UMIN is not relabeled as NCT, and the identifier is not treated as current registration-content verification.
External paper	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 30057059, DOI 10.1016/j.ctcp.2018.04.005 and UMIN000019409. PMCID is unrecorded; support and exact comparative CIs are unverified. UMIN is not relabeled as NCT, and the identifier is not treated as current registration-content verification.
Registration material	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 30057059, DOI 10.1016/j.ctcp.2018.04.005 and UMIN000019409. PMCID is unrecorded; support and exact comparative CIs are unverified. UMIN is not relabeled as NCT, and the identifier is not treated as current registration-content verification.
Protocol	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 30057059, DOI 10.1016/j.ctcp.2018.04.005 and UMIN000019409. PMCID is unrecorded; support and exact comparative CIs are unverified. UMIN is not relabeled as NCT, and the identifier is not treated as current registration-content verification.
Statistical analysis plan	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 30057059, DOI 10.1016/j.ctcp.2018.04.005 and UMIN000019409. PMCID is unrecorded; support and exact comparative CIs are unverified. UMIN is not relabeled as NCT, and the identifier is not treated as current registration-content verification.
Funding, product-support and COI material	Original bibliographic link or needed material; external public availability/access status unverified	Unread; access not attempted	Not read	Preserve original PMID 30057059, DOI 10.1016/j.ctcp.2018.04.005 and UMIN000019409. PMCID is unrecorded; support and exact comparative CIs are unverified. UMIN is not relabeled as NCT, and the identifier is not treated as current registration-content verification.

Report counts versus independent study counts

The parent, perceived-stress and cortisol subclaims share the four original reports. Direct cortisol reports are distinguished from background stress reports. Original report-specific groups are retained, but exact recruitment, cohort and registration relationships and unique trial, participant and event counts are unverified. Other sheets, arms and sessions are not added as new independent replications.

Review criteria

Version 1.0 · Criteria record date 2026-10-06. These are Chamgap's own application conditions, informed by the referenced methods. They do not constitute official assessment-tool certification or a new efficacy score.

N01 · What are the selected cortisol question and verdict?

Required materials and conditions: The existing subclaim, grade and explanation versus the parent verdict
Conditions for verification: Material for The existing subclaim, grade and explanation versus the parent verdict must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects The existing subclaim, grade and explanation versus the parent verdict with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N02 · Who participated in the cortisol trials?

Required materials and conditions: Trial-specific health, age, recruitment and baseline characteristics
Conditions for verification: Material for Trial-specific health, age, recruitment and baseline characteristics must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Trial-specific health, age, recruitment and baseline characteristics with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N03 · Is laughter itself the same as the combined program?

Required materials and conditions: Components involving laughter, clapping, breathing, movement and group participation
Conditions for verification: Material for Components involving laughter, clapping, breathing, movement and group participation must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Components involving laughter, clapping, breathing, movement and group participation with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N04 · How do acute, repeated and parent-program exposures differ?

Required materials and conditions: Trial-specific actual exposure, schedules and observation times
Conditions for verification: Material for Trial-specific actual exposure, schedules and observation times must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Trial-specific actual exposure, schedules and observation times with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N05 · What comparison do humorous video and reading provide?

Required materials and conditions: Control components, contact, emotion, breathing and direct contrasts
Conditions for verification: Material for Control components, contact, emotion, breathing and direct contrasts must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Control components, contact, emotion, breathing and direct contrasts with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N06 · What were the repeated trial's controls, expectations and contact?

Required materials and conditions: Correspondence of controls, social contact and expectations
Conditions for verification: Material for Correspondence of controls, social contact and expectations must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Correspondence of controls, social contact and expectations with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N07 · Were randomization and laboratory blinding verified?

Required materials and conditions: Allocation, concealment and collection, assay and analysis roles
Conditions for verification: Material for Allocation, concealment and collection, assay and analysis roles must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Allocation, concealment and collection, assay and analysis roles with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N08 · When were saliva samples repeatedly collected?

Required materials and conditions: Collection times, pre/post intervals, session windows and within-person repeats
Conditions for verification: Material for Collection times, pre/post intervals, session windows and within-person repeats must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Collection times, pre/post intervals, session windows and within-person repeats with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N09 · What were the assay method and concentration units?

Required materials and conditions: Assay, units, detection limits, batches and specimen quality
Conditions for verification: Material for Assay, units, detection limits, batches and specimen quality must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Assay, units, detection limits, batches and specimen quality with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N10 · Can baseline, final, change and between-arm effects be determined?

Required materials and conditions: Trial-, session- and arm-specific estimates and confidence intervals
Conditions for verification: Material for Trial-, session- and arm-specific estimates and confidence intervals must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Trial-, session- and arm-specific estimates and confidence intervals with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N11 · Do positive results in some sessions establish a consistent overall reduction?

Required materials and conditions: Session-specific results, selection, multiplicity and prespecified contrasts
Conditions for verification: Material for Session-specific results, selection, multiplicity and prespecified contrasts must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Session-specific results, selection, multiplicity and prespecified contrasts with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N12 · Does acute change over time establish a laughter-yoga-specific effect?

Required materials and conditions: Time effects, between-arm and group-by-time effects and prespecified comparisons
Conditions for verification: Material for Time effects, between-arm and group-by-time effects and prespecified comparisons must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Time effects, between-arm and group-by-time effects and prespecified comparisons with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N13 · Are DHEA and cortisol the same outcome?

Required materials and conditions: Separate biomarker analyses, denominators and results
Conditions for verification: Material for Separate biomarker analyses, denominators and results must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Separate biomarker analyses, denominators and results with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N14 · Is the total sample a valid saliva-analysis denominator?

Required materials and conditions: Allocation, completion and arm- and session-specific valid specimens and missingness
Conditions for verification: Material for Allocation, completion and arm- and session-specific valid specimens and missingness must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Allocation, completion and arm- and session-specific valid specimens and missingness with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N15 · How were missing and repeated data analyzed?

Required materials and conditions: Analysis populations, missing specimens, repeated-data models and adjustment
Conditions for verification: Material for Analysis populations, missing specimens, repeated-data models and adjustment must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Analysis populations, missing specimens, repeated-data models and adjustment with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N16 · Were cortisol hierarchy and registration prespecified?

Required materials and conditions: Primary/secondary outcomes, collection, analysis, registration and amendments
Conditions for verification: Material for Primary/secondary outcomes, collection, analysis, registration and amendments must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Primary/secondary outcomes, collection, analysis, registration and amendments with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N17 · Does a large PSS statistic establish a cortisol effect?

Required materials and conditions: Scope and role of the parent stress outcome
Conditions for verification: Material for Scope and role of the parent stress outcome must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Scope and role of the parent stress outcome with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N18 · Are conflicting abstract and main-text statements cortisol results?

Required materials and conditions: Attribution of statements and multivariate tests to their own endpoints
Conditions for verification: Material for Attribution of statements and multivariate tests to their own endpoints must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Attribution of statements and multivariate tests to their own endpoints with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N19 · Does a short-term biomarker change establish clinical meaning and persistence?

Required materials and conditions: Meaning of change, long-term outcomes and disorder outcomes
Conditions for verification: Material for Meaning of change, long-term outcomes and disorder outcomes must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Meaning of change, long-term outcomes and disorder outcomes with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N20 · Was independent replication under the same conditions established?

Required materials and conditions: The same biomarker, comparator, duration and report/cohort relationships
Conditions for verification: Material for The same biomarker, comparator, duration and report/cohort relationships must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects The same biomarker, comparator, duration and report/cohort relationships with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N21 · What were concurrent factors and collection environments?

Required materials and conditions: Research records of treatment, lifestyle, performance and collection conditions
Conditions for verification: Material for Research records of treatment, lifestyle, performance and collection conditions must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Supplementary
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Research records of treatment, lifestyle, performance and collection conditions with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N22 · What support and conflicts were disclosed in direct cortisol reports?

Required materials and conditions: Funding, training support, employee authors and conflicts
Conditions for verification: Material for Funding, training support, employee authors and conflicts must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Funding, training support, employee authors and conflicts with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N23 · Does a no-support declaration verify role separation and independence?

Required materials and conditions: Support/conflict declarations and instructor/assessor roles
Conditions for verification: Material for Support/conflict declarations and instructor/assessor roles must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Support/conflict declarations and instructor/assessor roles with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N24 · What context do other stress trials' registration and support provide?

Required materials and conditions: Unverified support, retrospective registration and the scope of originals
Conditions for verification: Material for Unverified support, retrospective registration and the scope of originals must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Unverified support, retrospective registration and the scope of originals with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N25 · How were harms collected and compared?

Required materials and conditions: Harms definitions, events, denominators, exposure, discontinuation and missingness
Conditions for verification: Material for Harms definitions, events, denominators, exposure, discontinuation and missingness must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Supplementary
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Harms definitions, events, denominators, exposure, discontinuation and missingness with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N26 · What are the boundaries for long-term and vulnerable-population harms and guidance?

Required materials and conditions: Populations, concurrent exposures, long-term harms and the original safety explanation
Conditions for verification: Material for Populations, concurrent exposures, long-term harms and the original safety explanation must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Supplementary
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Populations, concurrent exposures, long-term harms and the original safety explanation with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N27 · How are explanatory numbers, languages and parent axes retained?

Required materials and conditions: Original Korean/English explanations, parent precision and corrections
Conditions for verification: Material for Original Korean/English explanations, parent precision and corrections must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Original Korean/English explanations, parent precision and corrections with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

N28 · What was actually read, and what is the status of external materials?

Required materials and conditions: Reading status of stored summaries, originals, registration, plans and support
Conditions for verification: Material for Reading status of stored summaries, originals, registration, plans and support must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Requirement and applicability: Required
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Why this condition is needed: This condition connects Reading status of stored summaries, originals, registration, plans and support with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Referenced methods and the sections actually reviewed

M03 · Cochrane Handbook 6.5 (2024), chapter updated 2023-08

Official methodology · §3.1, §3.2.1–3.2.3, MECIR C5/C7/C13

Referenced principle: Clearly record the question, eligibility conditions and reasons for changes, and distinguish variations in interventions.
Extent of review: Existing methodological links were reused for production. External originals were not reread and methodological content was not reassessed.

M04 · Cochrane Handbook 6.5.1 (2025), chapter updated 2025-03

Official methodology · §4.5, §4.6.1–4.6.3, MECIR C42

Referenced principle: Distinguish studies from reports and link them; do not omit a study solely because results are unreported.
Extent of review: Existing methodological links were reused for production. External originals were not reread and methodological content was not reassessed.

M08 · Cochrane Handbook 6.5 (2024), chapter updated 2019-10

Official methodology · §8.1–8.2.3, Table 8.2.a

Referenced principle: Define the outcome and effect of interest, then examine randomization, deviations, missing data, measurement and selective reporting.
Extent of review: Existing methodological links were reused for production. External originals were not reread and methodological content was not reassessed.

M15 · Cochrane Handbook 6.5 (2024), chapter updated 2023-08

Official methodology · §15.2.4, §15.3.1–15.3.3

Referenced principle: Distinguish effect estimates, intervals, importance and applicability. Sample size alone does not determine precision.
Extent of review: Existing methodological links were reused for production. External originals were not reread and methodological content was not reassessed.

M19 · Cochrane Handbook 6.5 (2024), chapter updated 2019-10

Official methodology · §19.1.2, §19.2.1–19.2.4

Referenced principle: Harm questions may require different designs, populations and follow-up from efficacy questions. Case reports do not establish incidence rates.
Extent of review: Existing methodological links were reused for production. External originals were not reread and methodological content was not reassessed.

What the statuses mean

Met: A state used when required evidence is confirmed after user review. This production does not declare that review complete.
Partly verified: Some information in the stored summary is linked into the table. External comparison/content review is not complete.
Unverified: The article leaves this unverified, or required material is absent from the current stored summary. This is distinct from zero, absence or safety.
Review incomplete: User review pending.
Not reported: Not reported within the stored material actually read; not extended to the entire primary paper.
Inaccessible: Means an actual access failure. Access not attempted here is not assigned this state.
Not applicable: Not used as a substitute for an evidence gap.
Conflicting data: Requires an observed disagreement under the same comparison criteria; not automatically judged in this production.
Insufficient evidence for the conditions: Marks a gap in additional material required by the selected conditions; not a new efficacy grade.

Change history

2026-10-19 · Public page/material version 1.0

Public reader pages and material were prepared from the existing 28 items. No new clinical searches, verification-state changes or scoring were performed.

The stored-material review date (Not recorded) and original evidence cut-off (2026-09-07) were retained unchanged.

Researcher downloads

These materials provide the same evidence, scope and verification status as this page.

View all item details

N01 · What are the selected cortisol question and verdict?

Current findings: The selection is reduced salivary cortisol. Original subclaim C and the explanation of a weak 46-level signal with insufficient laughter specificity and session-to-session consistency are retained. The parent is nonclinical stress C50.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: The selection has no separate score, status or assessment axes. The explanatory number is not turned into a new structured score or filled with the parent score.
Required materials and conditions: The existing subclaim, grade and explanation versus the parent verdict
Materials to obtain first: The selection has no separate score, status or assessment axes. The explanatory number is not turned into a new structured score or filled with the parent score.
Further research: Material needed: The selection has no separate score, status or assessment axes. The explanatory number is not turned into a new structured score or filled with the parent score. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for The existing subclaim, grade and explanation versus the parent verdict must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects The existing subclaim, grade and explanation versus the parent verdict with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03 S04

N02 · Who participated in the cortisol trials?

Current findings: Ozturk included 75 first-year nursing students; Fujisawa included 120 healthy university students. The 70 psychiatric nurses belonged to a different stress trial.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Exact eligibility, sex, age, medication and lifestyle factors and baseline values in the direct cortisol trials are unverified.
Required materials and conditions: Trial-specific health, age, recruitment and baseline characteristics
Materials to obtain first: Exact eligibility, sex, age, medication and lifestyle factors and baseline values in the direct cortisol trials are unverified.
Further research: Material needed: Exact eligibility, sex, age, medication and lifestyle factors and baseline values in the direct cortisol trials are unverified. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Trial-specific health, age, recruitment and baseline characteristics must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Trial-specific health, age, recruitment and baseline characteristics with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03 S04

N03 · Is laughter itself the same as the combined program?

Current findings: The original program combines laughter with clapping, breathing, movement and group participation. It is not treated as a laughter-only effect, and no new practice method is provided.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Cortisol-trial-specific session components, instructors, contact and actual participation are unverified.
Required materials and conditions: Components involving laughter, clapping, breathing, movement and group participation
Materials to obtain first: Cortisol-trial-specific session components, instructors, contact and actual participation are unverified.
Further research: Material needed: Cortisol-trial-specific session components, instructors, contact and actual participation are unverified. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Components involving laughter, clapping, breathing, movement and group participation must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Components involving laughter, clapping, breathing, movement and group participation with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03 S04

N04 · How do acute, repeated and parent-program exposures differ?

Current findings: Fujisawa used an acute 3-arm design; Ozturk used 8 sessions. The recent positive program of 8 sessions of 30 minutes in the parent explanation belongs to the Bilgihan stress-trial context.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: The parent-program specification is not applied to other cortisol trials. Exposure, adherence and observation windows in direct trials are unverified.
Required materials and conditions: Trial-specific actual exposure, schedules and observation times
Materials to obtain first: The parent-program specification is not applied to other cortisol trials. Exposure, adherence and observation windows in direct trials are unverified.
Further research: Material needed: The parent-program specification is not applied to other cortisol trials. Exposure, adherence and observation windows in direct trials are unverified. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Trial-specific actual exposure, schedules and observation times must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Trial-specific actual exposure, schedules and observation times with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S01 S03 S04

N05 · What comparison do humorous video and reading provide?

Current findings: The trial in 120 healthy students had 3 arms: laughter yoga, humorous video and reading. Video results are not reassigned to laughter yoga.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Arm-specific expectations, contact, breathing and emotional components and exact between-arm contrasts are unverified.
Required materials and conditions: Control components, contact, emotion, breathing and direct contrasts
Materials to obtain first: Arm-specific expectations, contact, breathing and emotional components and exact between-arm contrasts are unverified.
Further research: Material needed: Arm-specific expectations, contact, breathing and emotional components and exact between-arm contrasts are unverified. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Control components, contact, emotion, breathing and direct contrasts must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Control components, contact, emotion, breathing and direct contrasts with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S04

N06 · What were the repeated trial's controls, expectations and contact?

Current findings: Ozturk is preserved as an 8-session randomized trial. The parent explanation describes insufficient active social controls as a limitation.
Verification status: Unverified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: The exact components, contact amount and expectation assessment of the Ozturk control are unverified. The parent's no-intervention label is not automatically assigned to this trial.
Required materials and conditions: Correspondence of controls, social contact and expectations
Materials to obtain first: The exact components, contact amount and expectation assessment of the Ozturk control are unverified. The parent's no-intervention label is not automatically assigned to this trial.
Further research: Material needed: The exact components, contact amount and expectation assessment of the Ozturk control are unverified. The parent's no-intervention label is not automatically assigned to this trial. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Correspondence of controls, social contact and expectations must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Correspondence of controls, social contact and expectations with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03

N07 · Were randomization and laboratory blinding verified?

Current findings: The direct cortisol reports are randomized trials. Parent concerns about lack of blinding and combined instructor/assessor roles are distinguished as context from the recent stress trial.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Trial-specific allocation procedures, assessment and laboratory blinding and responsible roles in the direct cortisol trials are unverified.
Required materials and conditions: Allocation, concealment and collection, assay and analysis roles
Materials to obtain first: Trial-specific allocation procedures, assessment and laboratory blinding and responsible roles in the direct cortisol trials are unverified.
Further research: Material needed: Trial-specific allocation procedures, assessment and laboratory blinding and responsible roles in the direct cortisol trials are unverified. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Allocation, concealment and collection, assay and analysis roles must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Allocation, concealment and collection, assay and analysis roles with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03 S04

N08 · When were saliva samples repeatedly collected?

Current findings: The original statements are retained: Ozturk was between-arm positive in only 3 of 8 sessions; Fujisawa showed acute cortisol change over time.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Collection times, pre/post-session intervals, repeat counts and daily conditions are unverified. These are not converted into daily averages or long-term reductions.
Required materials and conditions: Collection times, pre/post intervals, session windows and within-person repeats
Materials to obtain first: Collection times, pre/post-session intervals, repeat counts and daily conditions are unverified. These are not converted into daily averages or long-term reductions.
Further research: Material needed: Collection times, pre/post-session intervals, repeat counts and daily conditions are unverified. These are not converted into daily averages or long-term reductions. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Collection times, pre/post intervals, session windows and within-person repeats must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Collection times, pre/post intervals, session windows and within-person repeats with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03 S04

N09 · What were the assay method and concentration units?

Current findings: The endpoint of the direct reports is salivary cortisol.
Verification status: Unverified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Quantitative values, units, assay methods, detection limits, batches, specimen handling and quality criteria are unverified.
Required materials and conditions: Assay, units, detection limits, batches and specimen quality
Materials to obtain first: Quantitative values, units, assay methods, detection limits, batches, specimen handling and quality criteria are unverified.
Further research: Material needed: Quantitative values, units, assay methods, detection limits, batches, specimen handling and quality criteria are unverified. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Assay, units, detection limits, batches and specimen quality must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Assay, units, detection limits, batches and specimen quality with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03 S04

N10 · Can baseline, final, change and between-arm effects be determined?

Current findings: The stored summary contains mixed session results and changes over time; the original statement that exact between-arm confidence intervals are unverified is retained.
Verification status: Unverified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Arm-specific baseline and final values, changes, between-arm effects, confidence intervals and exact significance levels are unverified. No new effects or intervals are calculated.
Required materials and conditions: Trial-, session- and arm-specific estimates and confidence intervals
Materials to obtain first: Arm-specific baseline and final values, changes, between-arm effects, confidence intervals and exact significance levels are unverified. No new effects or intervals are calculated.
Further research: Material needed: Arm-specific baseline and final values, changes, between-arm effects, confidence intervals and exact significance levels are unverified. No new effects or intervals are calculated. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Trial-, session- and arm-specific estimates and confidence intervals must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Trial-, session- and arm-specific estimates and confidence intervals with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03 S04

N11 · Do positive results in some sessions establish a consistent overall reduction?

Current findings: The original Ozturk result is between-arm positivity in only 3 of 8 sessions.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Which sessions were positive, directions and magnitudes, exact results in other sessions, prespecification and multiplicity adjustment are unverified. This is not converted into consistent reduction across all sessions.
Required materials and conditions: Session-specific results, selection, multiplicity and prespecified contrasts
Materials to obtain first: Which sessions were positive, directions and magnitudes, exact results in other sessions, prespecification and multiplicity adjustment are unverified. This is not converted into consistent reduction across all sessions.
Further research: Material needed: Which sessions were positive, directions and magnitudes, exact results in other sessions, prespecification and multiplicity adjustment are unverified. This is not converted into consistent reduction across all sessions. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Session-specific results, selection, multiplicity and prespecified contrasts must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Session-specific results, selection, multiplicity and prespecified contrasts with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03

N12 · Does acute change over time establish a laughter-yoga-specific effect?

Current findings: The original Fujisawa statements of cortisol change over time and changes with humorous video are retained.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Exact laughter-yoga between-arm and group-by-time contrasts, net effects, confidence intervals and baseline adjustment are unverified. Time change is not promoted to a laughter-specific effect.
Required materials and conditions: Time effects, between-arm and group-by-time effects and prespecified comparisons
Materials to obtain first: Exact laughter-yoga between-arm and group-by-time contrasts, net effects, confidence intervals and baseline adjustment are unverified. Time change is not promoted to a laughter-specific effect.
Further research: Material needed: Exact laughter-yoga between-arm and group-by-time contrasts, net effects, confidence intervals and baseline adjustment are unverified. Time change is not promoted to a laughter-specific effect. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Time effects, between-arm and group-by-time effects and prespecified comparisons must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Time effects, between-arm and group-by-time effects and prespecified comparisons with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S04

N13 · Are DHEA and cortisol the same outcome?

Current findings: The original Fujisawa result retains both cortisol change and null DHEA findings.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: The biomarkers are not combined or converted into general physiological improvement. Exact values and outcome hierarchy for each biomarker are unverified.
Required materials and conditions: Separate biomarker analyses, denominators and results
Materials to obtain first: The biomarkers are not combined or converted into general physiological improvement. Exact values and outcome hierarchy for each biomarker are unverified.
Further research: Material needed: The biomarkers are not combined or converted into general physiological improvement. Exact values and outcome hierarchy for each biomarker are unverified. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Separate biomarker analyses, denominators and results must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Separate biomarker analyses, denominators and results with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S04

N14 · Is the total sample a valid saliva-analysis denominator?

Current findings: Original total samples of 75 in Ozturk and 120 in Fujisawa are retained.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Arm-specific allocation, completion, saliva denominators, specimen exclusions and time-specific missingness are unverified. Complete validity and session-specific denominators are not created.
Required materials and conditions: Allocation, completion and arm- and session-specific valid specimens and missingness
Materials to obtain first: Arm-specific allocation, completion, saliva denominators, specimen exclusions and time-specific missingness are unverified. Complete validity and session-specific denominators are not created.
Further research: Material needed: Arm-specific allocation, completion, saliva denominators, specimen exclusions and time-specific missingness are unverified. Complete validity and session-specific denominators are not created. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Allocation, completion and arm- and session-specific valid specimens and missingness must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Allocation, completion and arm- and session-specific valid specimens and missingness with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03 S04

N15 · How were missing and repeated data analyzed?

Current findings: The stored summary lacks details of quantitative analysis models and missing-data handling.
Verification status: Unverified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Missing saliva, correlations across sessions, specimen exclusions and adjustment for baseline, time and lifestyle factors are unverified.
Required materials and conditions: Analysis populations, missing specimens, repeated-data models and adjustment
Materials to obtain first: Missing saliva, correlations across sessions, specimen exclusions and adjustment for baseline, time and lifestyle factors are unverified.
Further research: Material needed: Missing saliva, correlations across sessions, specimen exclusions and adjustment for baseline, time and lifestyle factors are unverified. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Analysis populations, missing specimens, repeated-data models and adjustment must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Analysis populations, missing specimens, repeated-data models and adjustment with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03 S04

N16 · Were cortisol hierarchy and registration prespecified?

Current findings: The original records are retained: Ozturk registration was unable to be verified, and Fujisawa has UMIN000019409. The UMIN identifier also appears in the original citation annotation.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Registration originals and times, cortisol hierarchy, protocols, analysis plans and amendments are unread. An identifier alone does not certify prespecification.
Required materials and conditions: Primary/secondary outcomes, collection, analysis, registration and amendments
Materials to obtain first: Registration originals and times, cortisol hierarchy, protocols, analysis plans and amendments are unread. An identifier alone does not certify prespecification.
Further research: Material needed: Registration originals and times, cortisol hierarchy, protocols, analysis plans and amendments are unread. An identifier alone does not certify prespecification. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Primary/secondary outcomes, collection, analysis, registration and amendments must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Primary/secondary outcomes, collection, analysis, registration and amendments with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03 S04

N17 · Does a large PSS statistic establish a cortisol effect?

Current findings: Bilgihan's 70 participants, all completing at 35 versus 35, and PSS F(1,68)=251.56, P<.001, partial eta-squared=.787 are original parent stress-trial values.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: These statistics are not used as salivary cortisol effects or denominators or as disorder-treatment evidence. The original report's endpoint list does not include cortisol.
Required materials and conditions: Scope and role of the parent stress outcome
Materials to obtain first: These statistics are not used as salivary cortisol effects or denominators or as disorder-treatment evidence. The original report's endpoint list does not include cortisol.
Further research: Material needed: These statistics are not used as salivary cortisol effects or denominators or as disorder-treatment evidence. The original report's endpoint list does not include cortisol. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Scope and role of the parent stress outcome must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Scope and role of the parent stress outcome with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S01

N18 · Are conflicting abstract and main-text statements cortisol results?

Current findings: The original Yaş account retains 83 analyzed, 41 versus 42, coexisting abstract null and main-text multivariate interaction F=10.69, P<.001, and retrospective registration.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: These statistics and conflicts are not reassigned as direct cortisol effects or exact null effects. Original endpoints are well-being, PSS and self-efficacy.
Required materials and conditions: Attribution of statements and multivariate tests to their own endpoints
Materials to obtain first: These statistics and conflicts are not reassigned as direct cortisol effects or exact null effects. Original endpoints are well-being, PSS and self-efficacy.
Further research: Material needed: These statistics and conflicts are not reassigned as direct cortisol effects or exact null effects. Original endpoints are well-being, PSS and self-efficacy. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Attribution of statements and multivariate tests to their own endpoints must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Attribution of statements and multivariate tests to their own endpoints with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S02

N19 · Does a short-term biomarker change establish clinical meaning and persistence?

Current findings: The original explanation retains mixed cortisol results and gaps in exact between-arm confidence intervals as a separate weak signal.
Verification status: Unverified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Direct links to normalization, meaningful change, long-term persistence and depression/anxiety treatment are unverified.
Required materials and conditions: Meaning of change, long-term outcomes and disorder outcomes
Materials to obtain first: Direct links to normalization, meaningful change, long-term persistence and depression/anxiety treatment are unverified.
Further research: Material needed: Direct links to normalization, meaningful change, long-term persistence and depression/anxiety treatment are unverified. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Meaning of change, long-term outcomes and disorder outcomes must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Meaning of change, long-term outcomes and disorder outcomes with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03 S04

N20 · Was independent replication under the same conditions established?

Current findings: Single-exposure and 8-session direct reports are distinguished from separate stress reports. Group participation, breathing, attention and comedy effects are not combined as laughter-specific effects.
Verification status: Unverified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Independent confirmation under the same comparison conditions, heterogeneity and unique trial and participant totals are unverified.
Required materials and conditions: The same biomarker, comparator, duration and report/cohort relationships
Materials to obtain first: Independent confirmation under the same comparison conditions, heterogeneity and unique trial and participant totals are unverified.
Further research: Material needed: Independent confirmation under the same comparison conditions, heterogeneity and unique trial and participant totals are unverified. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for The same biomarker, comparator, duration and report/cohort relationships must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects The same biomarker, comparator, duration and report/cohort relationships with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S01 S02 S03 S04

N21 · What were concurrent factors and collection environments?

Current findings: The original explanation distinguishes the combined program from social-participation and breathing effects.
Verification status: Unverified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Medication, lifestyle, activities, collection environments and adherence in direct cortisol trials are unverified. Causes of biomarker changes are not newly established.
Required materials and conditions: Research records of treatment, lifestyle, performance and collection conditions
Materials to obtain first: Medication, lifestyle, activities, collection environments and adherence in direct cortisol trials are unverified. Causes of biomarker changes are not newly established.
Further research: Material needed: Medication, lifestyle, activities, collection environments and adherence in direct cortisol trials are unverified. Causes of biomarker changes are not newly established. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Research records of treatment, lifestyle, performance and collection conditions must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Research records of treatment, lifestyle, performance and collection conditions with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03 S04

N22 · What support and conflicts were disclosed in direct cortisol reports?

Current findings: The original record that funding in Ozturk and Fujisawa was unable to be verified is retained.
Verification status: Unverified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Evidence is lacking to establish the absence of support, affiliations, conflicts or involvement, or to certify independence.
Required materials and conditions: Funding, training support, employee authors and conflicts
Materials to obtain first: Evidence is lacking to establish the absence of support, affiliations, conflicts or involvement, or to certify independence.
Further research: Material needed: Evidence is lacking to establish the absence of support, affiliations, conflicts or involvement, or to certify independence. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Funding, training support, employee authors and conflicts must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Funding, training support, employee authors and conflicts with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S03 S04

N23 · Does a no-support declaration verify role separation and independence?

Current findings: Bilgihan's declaration of no external funding or conflicts is retained alongside combined instructor/assessor roles.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: The declaration is not converted into confirmation of all support, blinding or independence. Original support disclosures were not read in this work.
Required materials and conditions: Support/conflict declarations and instructor/assessor roles
Materials to obtain first: The declaration is not converted into confirmation of all support, blinding or independence. Original support disclosures were not read in this work.
Further research: Material needed: The declaration is not converted into confirmation of all support, blinding or independence. Original support disclosures were not read in this work. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Support/conflict declarations and instructor/assessor roles must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Support/conflict declarations and instructor/assessor roles with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S01

N24 · What context do other stress trials' registration and support provide?

Current findings: Yaş's unverified support and retrospective NCT06175936 registration and Bilgihan's NCT07015372 date-consistency concerns are retained separately.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: The registration, support and amendment originals are unread. They do not substitute for prespecification in direct cortisol trials.
Required materials and conditions: Unverified support, retrospective registration and the scope of originals
Materials to obtain first: The registration, support and amendment originals are unread. They do not substitute for prespecification in direct cortisol trials.
Further research: Material needed: The registration, support and amendment originals are unread. They do not substitute for prespecification in direct cortisol trials. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Unverified support, retrospective registration and the scope of originals must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Unverified support, retrospective registration and the scope of originals with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S01 S02

N25 · How were harms collected and compared?

Current findings: Parent safety is Unknown, with insufficient arm-specific adverse-event and long-term safety numbers. A biomarker reduction is not converted into safety.
Verification status: Unverified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Harms events, denominators, arm-specific comparisons, timing, missingness and discontinuation data are unverified.
Required materials and conditions: Harms definitions, events, denominators, exposure, discontinuation and missingness
Materials to obtain first: Harms events, denominators, arm-specific comparisons, timing, missingness and discontinuation data are unverified.
Further research: Material needed: Harms events, denominators, arm-specific comparisons, timing, missingness and discontinuation data are unverified. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Harms definitions, events, denominators, exposure, discontinuation and missingness must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Harms definitions, events, denominators, exposure, discontinuation and missingness with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S01 S02 S03 S04

N26 · What are the boundaries for long-term and vulnerable-population harms and guidance?

Current findings: The parent safety explanation is preserved as historical context. No new yoga or mental-health treatment instructions are created.
Verification status: Unverified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Long-term and vulnerable-population evidence, alternative causes and concurrent exposures are unverified. Unknown is not converted into safe or no events.
Required materials and conditions: Populations, concurrent exposures, long-term harms and the original safety explanation
Materials to obtain first: Long-term and vulnerable-population evidence, alternative causes and concurrent exposures are unverified. Unknown is not converted into safe or no events.
Further research: Material needed: Long-term and vulnerable-population evidence, alternative causes and concurrent exposures are unverified. Unknown is not converted into safe or no events. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Populations, concurrent exposures, long-term harms and the original safety explanation must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Populations, concurrent exposures, long-term harms and the original safety explanation with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S01 S02 S03 S04

N27 · How are explanatory numbers, languages and parent axes retained?

Current findings: Parent C50, Korean/English originals, unspecified precision, evidence date 2026-09-07, an empty corrections list and empty deliberation are retained. The subclaim C's 46-level and English C46 expressions are original explanation, with no separate score.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: Historical review and explanatory numbers are not inherited as new structured scores, current review or certification.
Required materials and conditions: Original Korean/English explanations, parent precision and corrections
Materials to obtain first: Historical review and explanatory numbers are not inherited as new structured scores, current review or certification.
Further research: Material needed: Historical review and explanatory numbers are not inherited as new structured scores, current review or certification. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Original Korean/English explanations, parent precision and corrections must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Original Korean/English explanations, parent precision and corrections with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S01 S02 S03 S04

N28 · What was actually read, and what is the status of external materials?

Current findings: The complete stored parent summary and bibliography were read for production. Material links, reading of stored summaries and unread external materials are distinguished.
Verification status: Partly verified
Materials actually read: Stored summary read; external papers, registration, protocols, analysis plans and support disclosures unread
Why unverified: Direct cortisol evidence and parent context were distinguished and transferred from the stored summary. Detailed original material is unverified and review of existing content remains incomplete. This does not establish completed clinical review or certification.
Reason for missing information: External papers, registration, protocols, analysis plans and support disclosures were unread and access was not attempted. Historical verification and URLs are not promoted to current reading.
Required materials and conditions: Reading status of stored summaries, originals, registration, plans and support
Materials to obtain first: External papers, registration, protocols, analysis plans and support disclosures were unread and access was not attempted. Historical verification and URLs are not promoted to current reading.
Further research: Material needed: External papers, registration, protocols, analysis plans and support disclosures were unread and access was not attempted. Historical verification and URLs are not promoted to current reading. This records needed materials; no new research was executed.
Remaining uncertainty: External originals are unread and review of existing content remains incomplete. Original grades, explanatory numbers, parent axes and historical narratives are not used as new verdicts, scores, current verification or independence certification.
Conditions for verification: Material for Reading status of stored summaries, originals, registration, plans and support must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.
Why this condition is needed: This condition connects Reading status of stored summaries, originals, registration, plans and support with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.
Application exceptions: Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.
Referenced methods: Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.

Related sources S01 S02 S03 S04