{
  "schema_version": "chamgap-public-criteria-1.0",
  "title": "Laughter Yoga × Reduced Salivary Cortisol · Evidence and Gaps Sheet",
  "language": "en",
  "translation_date": "2026-10-19",
  "criteria_version": "1.0",
  "criteria_date": "2026-10-06",
  "condition_origin": "Chamgap's own application conditions",
  "method_sources": [
    {
      "id": "M03",
      "version": "Cochrane Handbook 6.5 (2024), chapter updated 2023-08",
      "url": "https://www.cochrane.org/authors/handbooks-and-manuals/handbook/current/chapter-03",
      "section": "§3.1, §3.2.1–3.2.3, MECIR C5/C7/C13",
      "official_principle": "Clearly record the question, eligibility conditions and reasons for changes, and distinguish variations in interventions.",
      "review_level": "Existing methodological links were reused for production. External originals were not reread and methodological content was not reassessed."
    },
    {
      "id": "M04",
      "version": "Cochrane Handbook 6.5.1 (2025), chapter updated 2025-03",
      "url": "https://www.cochrane.org/authors/handbooks-and-manuals/handbook/current/chapter-04",
      "section": "§4.5, §4.6.1–4.6.3, MECIR C42",
      "official_principle": "Distinguish studies from reports and link them; do not omit a study solely because results are unreported.",
      "review_level": "Existing methodological links were reused for production. External originals were not reread and methodological content was not reassessed."
    },
    {
      "id": "M08",
      "version": "Cochrane Handbook 6.5 (2024), chapter updated 2019-10",
      "url": "https://www.cochrane.org/authors/handbooks-and-manuals/handbook/current/chapter-08",
      "section": "§8.1–8.2.3, Table 8.2.a",
      "official_principle": "Define the outcome and effect of interest, then examine randomization, deviations, missing data, measurement and selective reporting.",
      "review_level": "Existing methodological links were reused for production. External originals were not reread and methodological content was not reassessed."
    },
    {
      "id": "M15",
      "version": "Cochrane Handbook 6.5 (2024), chapter updated 2023-08",
      "url": "https://www.cochrane.org/authors/handbooks-and-manuals/handbook/current/chapter-15",
      "section": "§15.2.4, §15.3.1–15.3.3",
      "official_principle": "Distinguish effect estimates, intervals, importance and applicability. Sample size alone does not determine precision.",
      "review_level": "Existing methodological links were reused for production. External originals were not reread and methodological content was not reassessed."
    },
    {
      "id": "M19",
      "version": "Cochrane Handbook 6.5 (2024), chapter updated 2019-10",
      "url": "https://www.cochrane.org/authors/handbooks-and-manuals/handbook/current/chapter-19",
      "section": "§19.1.2, §19.2.1–19.2.4",
      "official_principle": "Harm questions may require different designs, populations and follow-up from efficacy questions. Case reports do not establish incidence rates.",
      "review_level": "Existing methodological links were reused for production. External originals were not reread and methodological content was not reassessed."
    }
  ],
  "status_dictionary": {
    "Met": "A state used when required evidence is confirmed after user review. This production does not declare that review complete.",
    "Partly verified": "Some information in the stored summary is linked into the table. External comparison/content review is not complete.",
    "Unverified": "The article leaves this unverified, or required material is absent from the current stored summary. This is distinct from zero, absence or safety.",
    "Review incomplete": "User review pending.",
    "Not reported": "Not reported within the stored material actually read; not extended to the entire primary paper.",
    "Inaccessible": "Means an actual access failure. Access not attempted here is not assigned this state.",
    "Not applicable": "Not used as a substitute for an evidence gap.",
    "Conflicting data": "Requires an observed disagreement under the same comparison criteria; not automatically judged in this production.",
    "Insufficient evidence for the conditions": "Marks a gap in additional material required by the selected conditions; not a new efficacy grade."
  },
  "requirements": [
    {
      "item_id": "N01",
      "question_type": "Evidence condition",
      "question": "What are the selected cortisol question and verdict?",
      "needed": "The existing subclaim, grade and explanation versus the parent verdict",
      "role": "Required",
      "role_reason": "This condition connects The existing subclaim, grade and explanation versus the parent verdict with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for The existing subclaim, grade and explanation versus the parent verdict must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: The selection has no separate score, status or assessment axes. The explanatory number is not turned into a new structured score or filled with the parent score. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N02",
      "question_type": "Evidence condition",
      "question": "Who participated in the cortisol trials?",
      "needed": "Trial-specific health, age, recruitment and baseline characteristics",
      "role": "Required",
      "role_reason": "This condition connects Trial-specific health, age, recruitment and baseline characteristics with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Trial-specific health, age, recruitment and baseline characteristics must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Exact eligibility, sex, age, medication and lifestyle factors and baseline values in the direct cortisol trials are unverified. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N03",
      "question_type": "Evidence condition",
      "question": "Is laughter itself the same as the combined program?",
      "needed": "Components involving laughter, clapping, breathing, movement and group participation",
      "role": "Required",
      "role_reason": "This condition connects Components involving laughter, clapping, breathing, movement and group participation with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Components involving laughter, clapping, breathing, movement and group participation must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Cortisol-trial-specific session components, instructors, contact and actual participation are unverified. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N04",
      "question_type": "Evidence condition",
      "question": "How do acute, repeated and parent-program exposures differ?",
      "needed": "Trial-specific actual exposure, schedules and observation times",
      "role": "Required",
      "role_reason": "This condition connects Trial-specific actual exposure, schedules and observation times with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Trial-specific actual exposure, schedules and observation times must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: The parent-program specification is not applied to other cortisol trials. Exposure, adherence and observation windows in direct trials are unverified. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N05",
      "question_type": "Evidence condition",
      "question": "What comparison do humorous video and reading provide?",
      "needed": "Control components, contact, emotion, breathing and direct contrasts",
      "role": "Required",
      "role_reason": "This condition connects Control components, contact, emotion, breathing and direct contrasts with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Control components, contact, emotion, breathing and direct contrasts must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Arm-specific expectations, contact, breathing and emotional components and exact between-arm contrasts are unverified. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N06",
      "question_type": "Evidence condition",
      "question": "What were the repeated trial's controls, expectations and contact?",
      "needed": "Correspondence of controls, social contact and expectations",
      "role": "Required",
      "role_reason": "This condition connects Correspondence of controls, social contact and expectations with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Correspondence of controls, social contact and expectations must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: The exact components, contact amount and expectation assessment of the Ozturk control are unverified. The parent's no-intervention label is not automatically assigned to this trial. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N07",
      "question_type": "Evidence condition",
      "question": "Were randomization and laboratory blinding verified?",
      "needed": "Allocation, concealment and collection, assay and analysis roles",
      "role": "Required",
      "role_reason": "This condition connects Allocation, concealment and collection, assay and analysis roles with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Allocation, concealment and collection, assay and analysis roles must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Trial-specific allocation procedures, assessment and laboratory blinding and responsible roles in the direct cortisol trials are unverified. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N08",
      "question_type": "Evidence condition",
      "question": "When were saliva samples repeatedly collected?",
      "needed": "Collection times, pre/post intervals, session windows and within-person repeats",
      "role": "Required",
      "role_reason": "This condition connects Collection times, pre/post intervals, session windows and within-person repeats with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Collection times, pre/post intervals, session windows and within-person repeats must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Collection times, pre/post-session intervals, repeat counts and daily conditions are unverified. These are not converted into daily averages or long-term reductions. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N09",
      "question_type": "Evidence condition",
      "question": "What were the assay method and concentration units?",
      "needed": "Assay, units, detection limits, batches and specimen quality",
      "role": "Required",
      "role_reason": "This condition connects Assay, units, detection limits, batches and specimen quality with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Assay, units, detection limits, batches and specimen quality must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Quantitative values, units, assay methods, detection limits, batches, specimen handling and quality criteria are unverified. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N10",
      "question_type": "Evidence condition",
      "question": "Can baseline, final, change and between-arm effects be determined?",
      "needed": "Trial-, session- and arm-specific estimates and confidence intervals",
      "role": "Required",
      "role_reason": "This condition connects Trial-, session- and arm-specific estimates and confidence intervals with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Trial-, session- and arm-specific estimates and confidence intervals must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Arm-specific baseline and final values, changes, between-arm effects, confidence intervals and exact significance levels are unverified. No new effects or intervals are calculated. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N11",
      "question_type": "Evidence condition",
      "question": "Do positive results in some sessions establish a consistent overall reduction?",
      "needed": "Session-specific results, selection, multiplicity and prespecified contrasts",
      "role": "Required",
      "role_reason": "This condition connects Session-specific results, selection, multiplicity and prespecified contrasts with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Session-specific results, selection, multiplicity and prespecified contrasts must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Which sessions were positive, directions and magnitudes, exact results in other sessions, prespecification and multiplicity adjustment are unverified. This is not converted into consistent reduction across all sessions. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N12",
      "question_type": "Evidence condition",
      "question": "Does acute change over time establish a laughter-yoga-specific effect?",
      "needed": "Time effects, between-arm and group-by-time effects and prespecified comparisons",
      "role": "Required",
      "role_reason": "This condition connects Time effects, between-arm and group-by-time effects and prespecified comparisons with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Time effects, between-arm and group-by-time effects and prespecified comparisons must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Exact laughter-yoga between-arm and group-by-time contrasts, net effects, confidence intervals and baseline adjustment are unverified. Time change is not promoted to a laughter-specific effect. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N13",
      "question_type": "Evidence condition",
      "question": "Are DHEA and cortisol the same outcome?",
      "needed": "Separate biomarker analyses, denominators and results",
      "role": "Required",
      "role_reason": "This condition connects Separate biomarker analyses, denominators and results with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Separate biomarker analyses, denominators and results must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: The biomarkers are not combined or converted into general physiological improvement. Exact values and outcome hierarchy for each biomarker are unverified. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N14",
      "question_type": "Evidence condition",
      "question": "Is the total sample a valid saliva-analysis denominator?",
      "needed": "Allocation, completion and arm- and session-specific valid specimens and missingness",
      "role": "Required",
      "role_reason": "This condition connects Allocation, completion and arm- and session-specific valid specimens and missingness with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Allocation, completion and arm- and session-specific valid specimens and missingness must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Arm-specific allocation, completion, saliva denominators, specimen exclusions and time-specific missingness are unverified. Complete validity and session-specific denominators are not created. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N15",
      "question_type": "Evidence condition",
      "question": "How were missing and repeated data analyzed?",
      "needed": "Analysis populations, missing specimens, repeated-data models and adjustment",
      "role": "Required",
      "role_reason": "This condition connects Analysis populations, missing specimens, repeated-data models and adjustment with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Analysis populations, missing specimens, repeated-data models and adjustment must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Missing saliva, correlations across sessions, specimen exclusions and adjustment for baseline, time and lifestyle factors are unverified. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N16",
      "question_type": "Evidence condition",
      "question": "Were cortisol hierarchy and registration prespecified?",
      "needed": "Primary/secondary outcomes, collection, analysis, registration and amendments",
      "role": "Required",
      "role_reason": "This condition connects Primary/secondary outcomes, collection, analysis, registration and amendments with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Primary/secondary outcomes, collection, analysis, registration and amendments must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Registration originals and times, cortisol hierarchy, protocols, analysis plans and amendments are unread. An identifier alone does not certify prespecification. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N17",
      "question_type": "Evidence condition",
      "question": "Does a large PSS statistic establish a cortisol effect?",
      "needed": "Scope and role of the parent stress outcome",
      "role": "Required",
      "role_reason": "This condition connects Scope and role of the parent stress outcome with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Scope and role of the parent stress outcome must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: These statistics are not used as salivary cortisol effects or denominators or as disorder-treatment evidence. The original report's endpoint list does not include cortisol. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N18",
      "question_type": "Evidence condition",
      "question": "Are conflicting abstract and main-text statements cortisol results?",
      "needed": "Attribution of statements and multivariate tests to their own endpoints",
      "role": "Required",
      "role_reason": "This condition connects Attribution of statements and multivariate tests to their own endpoints with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Attribution of statements and multivariate tests to their own endpoints must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: These statistics and conflicts are not reassigned as direct cortisol effects or exact null effects. Original endpoints are well-being, PSS and self-efficacy. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N19",
      "question_type": "Evidence condition",
      "question": "Does a short-term biomarker change establish clinical meaning and persistence?",
      "needed": "Meaning of change, long-term outcomes and disorder outcomes",
      "role": "Required",
      "role_reason": "This condition connects Meaning of change, long-term outcomes and disorder outcomes with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Meaning of change, long-term outcomes and disorder outcomes must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Direct links to normalization, meaningful change, long-term persistence and depression/anxiety treatment are unverified. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N20",
      "question_type": "Evidence condition",
      "question": "Was independent replication under the same conditions established?",
      "needed": "The same biomarker, comparator, duration and report/cohort relationships",
      "role": "Required",
      "role_reason": "This condition connects The same biomarker, comparator, duration and report/cohort relationships with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for The same biomarker, comparator, duration and report/cohort relationships must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Independent confirmation under the same comparison conditions, heterogeneity and unique trial and participant totals are unverified. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N21",
      "question_type": "Evidence condition",
      "question": "What were concurrent factors and collection environments?",
      "needed": "Research records of treatment, lifestyle, performance and collection conditions",
      "role": "Supplementary",
      "role_reason": "This condition connects Research records of treatment, lifestyle, performance and collection conditions with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Research records of treatment, lifestyle, performance and collection conditions must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Medication, lifestyle, activities, collection environments and adherence in direct cortisol trials are unverified. Causes of biomarker changes are not newly established. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N22",
      "question_type": "Evidence condition",
      "question": "What support and conflicts were disclosed in direct cortisol reports?",
      "needed": "Funding, training support, employee authors and conflicts",
      "role": "Required",
      "role_reason": "This condition connects Funding, training support, employee authors and conflicts with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Funding, training support, employee authors and conflicts must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Evidence is lacking to establish the absence of support, affiliations, conflicts or involvement, or to certify independence. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N23",
      "question_type": "Evidence condition",
      "question": "Does a no-support declaration verify role separation and independence?",
      "needed": "Support/conflict declarations and instructor/assessor roles",
      "role": "Required",
      "role_reason": "This condition connects Support/conflict declarations and instructor/assessor roles with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Support/conflict declarations and instructor/assessor roles must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: The declaration is not converted into confirmation of all support, blinding or independence. Original support disclosures were not read in this work. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N24",
      "question_type": "Evidence condition",
      "question": "What context do other stress trials' registration and support provide?",
      "needed": "Unverified support, retrospective registration and the scope of originals",
      "role": "Required",
      "role_reason": "This condition connects Unverified support, retrospective registration and the scope of originals with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Unverified support, retrospective registration and the scope of originals must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: The registration, support and amendment originals are unread. They do not substitute for prespecification in direct cortisol trials. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N25",
      "question_type": "Evidence condition",
      "question": "How were harms collected and compared?",
      "needed": "Harms definitions, events, denominators, exposure, discontinuation and missingness",
      "role": "Supplementary",
      "role_reason": "This condition connects Harms definitions, events, denominators, exposure, discontinuation and missingness with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Harms definitions, events, denominators, exposure, discontinuation and missingness must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Harms events, denominators, arm-specific comparisons, timing, missingness and discontinuation data are unverified. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N26",
      "question_type": "Evidence condition",
      "question": "What are the boundaries for long-term and vulnerable-population harms and guidance?",
      "needed": "Populations, concurrent exposures, long-term harms and the original safety explanation",
      "role": "Supplementary",
      "role_reason": "This condition connects Populations, concurrent exposures, long-term harms and the original safety explanation with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Populations, concurrent exposures, long-term harms and the original safety explanation must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Long-term and vulnerable-population evidence, alternative causes and concurrent exposures are unverified. Unknown is not converted into safe or no events. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N27",
      "question_type": "Evidence condition",
      "question": "How are explanatory numbers, languages and parent axes retained?",
      "needed": "Original Korean/English explanations, parent precision and corrections",
      "role": "Required",
      "role_reason": "This condition connects Original Korean/English explanations, parent precision and corrections with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Original Korean/English explanations, parent precision and corrections must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: Historical review and explanatory numbers are not inherited as new structured scores, current review or certification. This records needed materials; no new research was executed."
    },
    {
      "item_id": "N28",
      "question_type": "Evidence condition",
      "question": "What was actually read, and what is the status of external materials?",
      "needed": "Reading status of stored summaries, originals, registration, plans and support",
      "role": "Required",
      "role_reason": "This condition connects Reading status of stored summaries, originals, registration, plans and support with direct salivary cortisol trials, populations, combined programs, actual controls, collection, units, timing, denominators, models, prespecification, between-arm effects or harms, distinguishing parent stress reports.",
      "application_exception": "Distinguish original subclaim C, explanatory 46-level wording and absent separate score/status/axes from parent C50 and Unknown safety. Positivity in some sessions, acute time changes and video-related changes are not converted into consistent overall reduction or laughter-specific effects. Parent PSS/multivariate statistics, headline samples, programs and blinding context are not reassigned as cortisol values, denominators or procedures. No new effects, confidence intervals, rates, clinical totals, independence/safety certification or practice instructions are created.",
      "criterion": "Material for Reading status of stored summaries, originals, registration, plans and support must be linked to the relevant cortisol trial, session, arm and outcome, distinguishing what the stored summary records from unread and unverified external material.",
      "basis": "Preserved C/E/H application conditions concerning questions, populations, exposure, comparators, reports versus trials, outcomes, denominators and harms were referenced.",
      "condition_origin": "These are in-house application conditions preserved for the existing subclaim. They do not establish preregistration, official-tool certification or completed individual content review.",
      "proposed_research": "Material needed: External papers, registration, protocols, analysis plans and support disclosures were unread and access was not attempted. Historical verification and URLs are not promoted to current reading. This records needed materials; no new research was executed."
    }
  ]
}