{ "schema_version": "chamgap-public-evidence-check-1.0", "public_id": "v2941-s1", "verdict_number": "2941", "title": "Two-year caloric restriction × reduced PC PhenoAge and PC GrimAge: a checklist of measure-specific evidence gaps", "view_version": "1.0", "view_created_date": "2026-10-11", "criteria_version": "1.0", "criteria_date": "2026-10-06", "review_date": null, "legacy_evidence_date": "2026-08-26", "review_scope": { "materials_read": "Existing review summaries and stored records of methodological application", "clinical_papers_directly_read": false, "new_clinical_search": false, "limitation": "The complete existing review summary was read for production, linking existing URLs and identifiers. External abstracts, full texts, registration, protocols and SAP were not newly read or checked.", "date_meaning": "This is the date the stored materials were checked, not the date of a new search of the clinical literature." }, "claim_context": { "population": "Healthy non-obese adults in CALERIE; three US sites; detailed eligibility not read", "intervention": "Historical two-year caloric-restriction study intervention; not personal calorie, diet or weight-loss instructions", "dose": "Original target of 25% and achieved average of about 12% preserved separately; individual and time-specific exposure details not read", "comparator": "Parent randomized control group; specific control diet, support and concurrent management not read", "endpoint": "Selected reductions in PC PhenoAge and PC GrimAge; unverified measure-specific values and time points, and DunedinPACE preserved separately", "duration": "Two-year study intervention; details of DunedinPACE at 12 and 24 months and time-specific PC outcomes unverified; post-intervention follow-up unverified", "excluded_extensions": [ "Recasting a DNA measure as clinical rejuvenation, aging in general, disease, disability, death, lifespan or independent living", "Treating target exposure and the achieved average as identical", "Summing time points, measures, the 197-person DNA denominator or sibling sheets as new independent evidence", "Personal calorie, dietary, weight-loss or treatment instructions" ] }, "requirements": [ { "item_id": "N01", "question_type": "Comparative efficacy", "question": "Are the original PC PhenoAge and PC GrimAge claim and subclaim assessment separated from the parent slowing context?", "needed": "Records of the subclaim's original wording, grade and note and the parent context", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Records of the subclaim's original wording, grade and note and the parent context", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Connect additional original material for each measure separately to the subclaim question and parent context.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original subclaim is 'Reduced PC PhenoAge and PC GrimAge', with grade D and a note that neither measure changed significantly. The parent overall assessment, including DunedinPACE, is C54 with Unknown safety.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. The subclaim has no score, status or axes; parent values are not used to fill them. No new assessment is made.", "missingness": "The subclaim has no score, status or axes; parent values are not used to fill them. No new assessment is made.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "The subclaim has no score, status or axes; parent values are not used to fill them. No new assessment is made." }, { "item_id": "N02", "question_type": "Comparative efficacy", "question": "Do the two PC measures concern healthy non-obese adults?", "needed": "Eligibility, baseline condition, age, BMI and group denominators", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Eligibility, baseline condition, age, BMI and group denominators", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original population and baseline characteristics and numbers analyzed for each measure.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original paper is a post hoc analysis of 220 healthy non-obese adults in CALERIE at three US sites.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. Findings are not automatically extended to direct effects in aging-related disease, obesity, other ages or frail populations. Detailed eligibility and baseline values have not been read.", "missingness": "Findings are not automatically extended to direct effects in aging-related disease, obesity, other ages or frail populations. Detailed eligibility and baseline values have not been read.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "Findings are not automatically extended to direct effects in aging-related disease, obesity, other ages or frail populations. Detailed eligibility and baseline values have not been read." }, { "item_id": "N03", "question_type": "Comparative efficacy", "question": "Are the 25% target and about 12% achieved restriction distinguished as study exposures?", "needed": "Original plans, achieved restriction, duration, adherence and measurements", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Original plans, achieved restriction, duration, adherence and measurements", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original exposure measurements, individual adherence, restriction achieved over time and support records.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original target is recorded as 25%, with average achieved restriction of about 12%.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. These are historical study conditions rather than personal calorie or weight-loss targets. The two PC findings are not assumed to be effects of the target exposure.", "missingness": "These are historical study conditions rather than personal calorie or weight-loss targets. The two PC findings are not assumed to be effects of the target exposure.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "These are historical study conditions rather than personal calorie or weight-loss targets. The two PC findings are not assumed to be effects of the target exposure." }, { "item_id": "N04", "question_type": "Comparative efficacy", "question": "Is the actual comparison of control diets, support and concurrent changes available?", "needed": "Actual diets, contact, support and concurrent management by group", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Actual diets, contact, support and concurrent management by group", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original intervention, control, support, achieved-exposure and concurrent-change material.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The parent's randomized two-year caloric-restriction context is preserved.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. Detailed control diets, exposures, contact and support are absent from the original summary. Equivalent nutrient composition or weight changes are not invented.", "missingness": "Detailed control diets, exposures, contact and support are absent from the original summary. Equivalent nutrient composition or weight changes are not invented.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "Detailed control diets, exposures, contact and support are absent from the original summary. Equivalent nutrient composition or weight changes are not invented." }, { "item_id": "N05", "question_type": "Comparative efficacy", "question": "Are 220 randomized participants and the 197-person DNA analysis time-specific PC denominators?", "needed": "Group allocation, DNA samples and analysis denominators by measure and time", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Group allocation, DNA samples and analysis denominators by measure and time", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain group samples and analysis flows and denominators by time and measure.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "There were 220 participants randomized 2:1; the DNA analysis included 197 participants with baseline and at least one follow-up sample.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. Group sizes are not calculated from 2:1 allocation. The 197 participants are not assumed to be the denominator for all PC outcomes at 12 or 24 months.", "missingness": "Group sizes are not calculated from 2:1 allocation. The 197 participants are not assumed to be the denominator for all PC outcomes at 12 or 24 months.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "Group sizes are not calculated from 2:1 allocation. The 197 participants are not assumed to be the denominator for all PC outcomes at 12 or 24 months." }, { "item_id": "N06", "question_type": "Comparative efficacy", "question": "Are gaps in DNA sample selection, missingness, follow-up and models preserved?", "needed": "Missingness, selection, analysis populations, sensitivity analyses and models by measure", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Missingness, selection, analysis populations, sensitivity analyses and models by measure", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original DNA selection, missingness, models and sensitivity material.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original account requires baseline and at least one follow-up sample for DNA inclusion.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. Group exclusions, time-specific missingness, ITT, data processing and sensitivity analyses have not been read. New attrition rates or differences are not calculated.", "missingness": "Group exclusions, time-specific missingness, ITT, data processing and sensitivity analyses have not been read. New attrition rates or differences are not calculated.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "Group exclusions, time-specific missingness, ITT, data processing and sensitivity analyses have not been read. New attrition rates or differences are not calculated." }, { "item_id": "N07", "question_type": "Comparative efficacy", "question": "Are the definition, units and original individual effects of PC PhenoAge directly available?", "needed": "Definitions, units, time points, denominators, effects and intervals for individual measures", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Definitions, units, time points, denominators, effects and intervals for individual measures", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original PC PhenoAge definitions, effects, CIs, P values and denominators by time.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "PC PhenoAge is one of the three original measures and is recorded as not significantly changed.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. The endpoints of the d −0.04 to 0.05 range in the original rationale are not assigned as a particular time-specific PC PhenoAge effect. Individual CIs, P values and unit details have not been read.", "missingness": "The endpoints of the d −0.04 to 0.05 range in the original rationale are not assigned as a particular time-specific PC PhenoAge effect. Individual CIs, P values and unit details have not been read.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "The endpoints of the d −0.04 to 0.05 range in the original rationale are not assigned as a particular time-specific PC PhenoAge effect. Individual CIs, P values and unit details have not been read." }, { "item_id": "N08", "question_type": "Comparative efficacy", "question": "Are the definition, units and original individual effects of PC GrimAge directly available?", "needed": "Definitions, units, time points, denominators, effects and intervals for individual measures", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Definitions, units, time points, denominators, effects and intervals for individual measures", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original PC GrimAge definitions, effects, CIs, P values and denominators by time.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "PC GrimAge is also one of the three original measures and is recorded as not significantly changed.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. The endpoints of the d range are not assigned as a particular PC GrimAge effect. Individual CIs, P values, denominators and algorithm definitions have not been read.", "missingness": "The endpoints of the d range are not assigned as a particular PC GrimAge effect. Individual CIs, P values, denominators and algorithm definitions have not been read.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "The endpoints of the d range are not assigned as a particular PC GrimAge effect. Individual CIs, P values, denominators and algorithm definitions have not been read." }, { "item_id": "N09", "question_type": "Comparative efficacy", "question": "Are the d range and P threshold presented jointly for the two measures distinguished from individual values?", "needed": "Detailed tables of original effects, intervals and tests by measure and time", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Detailed tables of original effects, intervals and tests by measure and time", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original tables of individual effects, CIs, tests, models and denominators.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original account describes the two PC measures as null, with d −0.04 to 0.05 and P>0.15.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. The range is not converted into a new mean, point estimates for each measure, exact P values or CIs. Zero effects or equivalence are not certified.", "missingness": "The range is not converted into a new mean, point estimates for each measure, exact P values or CIs. Zero effects or equivalence are not certified.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "The range is not converted into a new mean, point estimates for each measure, exact P values or CIs. Zero effects or equivalence are not certified." }, { "item_id": "N10", "question_type": "Comparative efficacy", "question": "Are PC outcomes at 12 and 24 months preserved separately by time?", "needed": "PC values, changes, between-group effects and denominators at each time", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "PC values, changes, between-group effects and denominators at each time", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original tables and analysis denominators for both PC measures at each time.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original rationale gives DunedinPACE results at 12 and 24 months specifically, but describes the two PC measures as null only within a summary range.", "condition_status": "Unverified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. Time-specific DunedinPACE d values, CIs and P values are not copied as PC values. Individual time-specific PC values remain unverified.", "missingness": "Time-specific DunedinPACE d values, CIs and P values are not copied as PC values. Individual time-specific PC values remain unverified.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "Time-specific DunedinPACE d values, CIs and P values are not copied as PC values. Individual time-specific PC values remain unverified." }, { "item_id": "N11", "question_type": "Comparative efficacy", "question": "Has the positive DunedinPACE signal been kept separate from PC reductions?", "needed": "Results, units, time points and direct original effects by measure", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Results, units, time points and direct original effects by measure", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Connect original effects, intervals, time points and models separately by measure.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original DunedinPACE signal is preserved: d=−0.29 (95% CI −0.45 to −0.13) at 12 months, d=−0.25 (−0.41 to −0.09) at 24 months, original P<0.003 and roughly 2~3% slowing.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. This measure's slowing is not used to fill in reductions in PC PhenoAge or PC GrimAge. A 24-month CI level or individual P values are not newly added.", "missingness": "This measure's slowing is not used to fill in reductions in PC PhenoAge or PC GrimAge. A 24-month CI level or individual P values are not newly added.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "This measure's slowing is not used to fill in reductions in PC PhenoAge or PC GrimAge. A 24-month CI level or individual P values are not newly added." }, { "item_id": "N12", "question_type": "Comparative efficacy", "question": "Do the PC age measures and DunedinPACE pace measure quantify the same thing?", "needed": "Purpose, definitions, units, validation and applicability of each measure", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Purpose, definitions, units, validation and applicability of each measure", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original methods, units and validation material separately for each measure.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original account separates three DNA-methylation clocks or measures and describes DunedinPACE as a surrogate pace-of-aging measure.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. Pace changes, PC reductions and clinical aging itself are not combined as the same value. Exact measure-specific definitions, units and validation have not been read.", "missingness": "Pace changes, PC reductions and clinical aging itself are not combined as the same value. Exact measure-specific definitions, units and validation have not been read.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "Pace changes, PC reductions and clinical aging itself are not combined as the same value. Exact measure-specific definitions, units and validation have not been read." }, { "item_id": "N13", "question_type": "Comparative efficacy", "question": "Are DNA laboratory methods, batches, normalization and PC calculation directly preserved?", "needed": "Original methods for samples, platforms, batches, normalization and PC calculation", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Original methods for samples, platforms, batches, normalization and PC calculation", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original laboratory, measure-calculation and data-processing methods.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The design is a post hoc DNA-methylation analysis; the original account records laboratory analyst blinding.", "condition_status": "Unverified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. PC algorithm, measurement, batch and normalization details are not invented, and new PC scores are not calculated from original data.", "missingness": "PC algorithm, measurement, batch and normalization details are not invented, and new PC scores are not calculated from original data.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "PC algorithm, measurement, batch and normalization details are not invented, and new PC scores are not calculated from original data." }, { "item_id": "N14", "question_type": "Comparative efficacy", "question": "Are the post hoc analysis and the parent's prespecified clinical primary plan separated?", "needed": "Original registration, protocol, SAP, analysis selection, changes and hierarchy", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Original registration, protocol, SAP, analysis selection, changes and hierarchy", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain parent plans and records of post hoc measure selection, hierarchy and changes.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The paper is explicitly post hoc, and the original account states that this was not the parent's prespecified clinical primary endpoint.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. The two PC measures are not newly designated as prespecified clinical coprimary endpoints. Registration and SAP remain unread in this production.", "missingness": "The two PC measures are not newly designated as prespecified clinical coprimary endpoints. Registration and SAP remain unread in this production.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "The two PC measures are not newly designated as prespecified clinical coprimary endpoints. Registration and SAP remain unread in this production." }, { "item_id": "N15", "question_type": "Comparative efficacy", "question": "Are the split findings across three key measures and plans for multiple time points and tests preserved?", "needed": "Original plans for three measures, time points, effects of interest, multiplicity and selective reporting", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Original plans for three measures, time points, effects of interest, multiplicity and selective reporting", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain tests and adjustments by measure and time, original outcome lists and selective-reporting records.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original assessment that only one of three measures was positive and the parent's split-key-measures flag set to true are preserved.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. The original split-results axis is not converted into a prespecified clinical primary designation. Adjustments for nonsignificant PC findings or positive DunedinPACE findings are not invented.", "missingness": "The original split-results axis is not converted into a prespecified clinical primary designation. Adjustments for nonsignificant PC findings or positive DunedinPACE findings are not invented.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "The original split-results axis is not converted into a prespecified clinical primary designation. Adjustments for nonsignificant PC findings or positive DunedinPACE findings are not invented." }, { "item_id": "N16", "question_type": "Comparative efficacy", "question": "Are null or nonsignificant findings distinguished from exclusion of a clinically important effect or equivalence?", "needed": "Individual CIs, validated thresholds and prespecified success boundaries", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Individual CIs, validated thresholds and prespecified success boundaries", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original CIs, clinical-importance validation and prespecified plans for each PC measure.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original account states that there was no clinical minimum-effect threshold or prespecified success boundary and records null findings for both PC measures.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. P>0.15 without individual CIs does not newly certify exclusion of all meaningful reductions or equivalence.", "missingness": "P>0.15 without individual CIs does not newly certify exclusion of all meaningful reductions or equivalence.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "P>0.15 without individual CIs does not newly certify exclusion of all meaningful reductions or equivalence." }, { "item_id": "N17", "question_type": "Comparative efficacy", "question": "Are blinding and allocation procedures recorded at their actual measure-specific scope?", "needed": "Parent allocation, laboratory analysts, statistical analysis and measurement methods", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Parent allocation, laboratory analysts, statistical analysis and measurement methods", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original allocation, laboratory and analyst roles and blinding procedures.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original Korean account says analysts were unaware of allocation; the English account specifies 'laboratory analysts blinded'. The 2:1 randomized design is preserved.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. Participant, intervention-provider or all-analyst blinding and allocation concealment are not described as newly verified.", "missingness": "Participant, intervention-provider or all-analyst blinding and allocation concealment are not described as newly verified.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "Participant, intervention-provider or all-analyst blinding and allocation concealment are not described as newly verified." }, { "item_id": "N18", "question_type": "Comparative efficacy", "question": "Is the relationship between achieved exposure, weight or nutrient changes and PC effects directly verified?", "needed": "Achieved restriction, adherence, concurrent changes and prespecified mediation or effects of interest", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Achieved restriction, adherence, concurrent changes and prespecified mediation or effects of interest", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original achieved exposure, adherence, concurrent changes, models and plans.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original information that target and achieved average restriction differed is preserved.", "condition_status": "Unverified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. PC effects by achieved restriction, mechanisms and causal relationships with weight changes are not newly calculated or confirmed.", "missingness": "PC effects by achieved restriction, mechanisms and causal relationships with weight changes are not newly calculated or confirmed.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "PC effects by achieved restriction, mechanisms and causal relationships with weight changes are not newly calculated or confirmed." }, { "item_id": "N19", "question_type": "Comparative efficacy", "question": "Do gaps remain concerning post-intervention persistence, later measures and mechanisms?", "needed": "Follow-up duration, measures, denominators, original effects and direct mechanism measurements", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Follow-up duration, measures, denominators, original effects and direct mechanism measurements", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain direct post-intervention measures, models, denominators and follow-up material.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original intervention lasted two years, and the stored summary has no quantitative post-intervention PC persistence effects.", "condition_status": "Unverified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. Post-intervention PC reductions, rebound or all-mechanism effects are not invented.", "missingness": "Post-intervention PC reductions, rebound or all-mechanism effects are not invented.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "Post-intervention PC reductions, rebound or all-mechanism effects are not invented." }, { "item_id": "N20", "question_type": "Comparative efficacy", "question": "Do null PC findings establish null results for all clinical aging, disease or lifespan outcomes?", "needed": "Direct clinical endpoints, follow-up, events, denominators and effects", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Direct clinical endpoints, follow-up, events, denominators and effects", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Direct events, long-term follow-up and original effects are needed for each clinical question.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original account states that reduced disease, disability or death was not measured and healthy lifespan was not answered.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. Null PC findings are not recast as null clinical effects overall; the DunedinPACE signal is not recast as benefits for rejuvenation, lifespan or independent living.", "missingness": "Null PC findings are not recast as null clinical effects overall; the DunedinPACE signal is not recast as benefits for rejuvenation, lifespan or independent living.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "Null PC findings are not recast as null clinical effects overall; the DunedinPACE signal is not recast as benefits for rejuvenation, lifespan or independent living." }, { "item_id": "N21", "question_type": "Comparative efficacy", "question": "Are the same CALERIE report, measures and time points kept from being counted as new independent replication?", "needed": "Relationships among the parent trial, post hoc report, same participants and sibling sheets", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Relationships among the parent trial, post hoc report, same participants and sibling sheets", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Connect additional reports through original trial identifiers while retaining same-population relationships.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "Both subclaim sheets share the same post hoc paper, 220 randomized participants and 197-person DNA data.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. Different measures, time points or sheets are not summed as independent trials or new participants. Final unique trial, participant and independent counts remain unrecorded.", "missingness": "Different measures, time points or sheets are not summed as independent trials or new participants. Final unique trial, participant and independent counts remain unrecorded.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "Different measures, time points or sheets are not summed as independent trials or new participants. Final unique trial, participant and independent counts remain unrecorded." }, { "item_id": "N22", "question_type": "Comparative efficacy", "question": "Are funding, DunedinPACE inventors and commercial licensing preserved alongside the PC findings?", "needed": "Funding, invention, licensing, affiliations, provision and analysis roles", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Funding, invention, licensing, affiliations, provision and analysis roles", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original funding, measure-related disclosures, affiliations, analysis and manuscript roles.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "Support from NIA, American Brain Foundation, CIHR and others, authorship by DunedinPACE inventors D.L. Corcoran and D.W. Belsky, and commercial licensing are preserved.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. The same invention or licensing interests are not newly added for the PC measures. Product or service provision, employee affiliations and outside-analysis details have not been read.", "missingness": "The same invention or licensing interests are not newly added for the PC measures. Product or service provision, employee affiliations and outside-analysis details have not been read.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "The same invention or licensing interests are not newly added for the PC measures. Product or service provision, employee affiliations and outside-analysis details have not been read." }, { "item_id": "N23", "question_type": "Supplementary harm evidence", "question": "Are absent safety reporting in this analysis and parent-trial harms separated?", "needed": "Analysis-specific and parent-trial harm plans, collection, events by group and denominators", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Analysis-specific and parent-trial harm plans, collection, events by group and denominators", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original parent-trial harm collection, events, exposure, discontinuation and denominators.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "This post hoc analysis did not report safety as an endpoint, and original safety is Unknown.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. Null PC findings or positive DunedinPACE findings are not used as evidence of safety. They are not extended to absence of all harms in the parent CALERIE trial.", "missingness": "Null PC findings or positive DunedinPACE findings are not used as evidence of safety. They are not extended to absence of all harms in the parent CALERIE trial.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "Null PC findings or positive DunedinPACE findings are not used as evidence of safety. They are not extended to absence of all harms in the parent CALERIE trial." }, { "item_id": "N24", "question_type": "Supplementary harm evidence", "question": "What gaps concern achieved two-year exposure, rare harms, discontinuation and long-term follow-up?", "needed": "Direct harms by group exposure, severity, discontinuation and duration", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Direct harms by group exposure, severity, discontinuation and duration", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Obtain original trial and follow-up harm, exposure, discontinuation and rare-event material.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original account explicitly states that this analysis cannot determine harms of long-term caloric restriction.", "condition_status": "Unverified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. Harm counts, rates, CIs or specific nutritional harms outside the original account are not invented. Null results for two measures are not assumed to mean no harms.", "missingness": "Harm counts, rates, CIs or specific nutritional harms outside the original account are not invented. Null results for two measures are not assumed to mean no harms.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "Harm counts, rates, CIs or specific nutritional harms outside the original account are not invented. Null results for two measures are not assumed to mean no harms." }, { "item_id": "N25", "question_type": "Supplementary harm evidence", "question": "Are harm replication, applicability and instructions for other populations or dietary exposures distinguished?", "needed": "Direct effects or harms and report relationships by population and exposure", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Direct effects or harms and report relationships by population and exposure", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Connect direct comparison and harm material for new populations or exposures separately.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The current material concerns historical two-year study conditions in healthy non-obese adults.", "condition_status": "Unverified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. Effects or harms for other populations or dietary methods are not newly confirmed; personal calorie, diet, weight-loss or treatment guidance is not created.", "missingness": "Effects or harms for other populations or dietary methods are not newly confirmed; personal calorie, diet, weight-loss or treatment guidance is not created.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "Effects or harms for other populations or dietary methods are not newly confirmed; personal calorie, diet, weight-loss or treatment guidance is not created." }, { "item_id": "N26", "question_type": "Comparative efficacy", "question": "Are the stored summary and actual access to external abstracts, full text, registration and SAP distinguished?", "needed": "Original links, registration identifiers, preserved original material and material-specific access records", "role": "Required", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Original links, registration identifiers, preserved original material and material-specific access records", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Connect provided original material with its actual material-specific access status.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The original PubMed link and NCT00427193 are preserved, and the entire parent summary was read for production.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. A registration URL is not newly constructed, and all external materials remain unread. Separate links, screens and content review were not checked during original production.", "missingness": "A registration URL is not newly constructed, and all external materials remain unread. Separate links, screens and content review were not checked during original production.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "A registration URL is not newly constructed, and all external materials remain unread. Separate links, screens and content review were not checked during original production." }, { "item_id": "N27", "question_type": "Comparative efficacy", "question": "Are the reference date, Korean and English text, empty values, subclaim and parent values, and production status preserved?", "needed": "Distinction among original text, assessment records, dates, corrections, candidate history and review status", "role": "Supplementary", "role_reason": "An individual condition for recording the selected measure, time, original values and applicability limits", "application_exception": "Unread external materials, other measures or out-of-scope outcomes do not establish that a condition is met, an effect is zero or safety is established.", "criterion": "Distinction among original text, assessment records, dates, corrections, candidate history and review status", "basis": "Reduced PC PhenoAge and PC GrimAge", "condition_origin": "Question-specific application conditions; existing content review remains incomplete and common definitions are not automatically fulfilled", "proposed_research": "Proposal: Reflect additional materials and decisions through preservation of original wording and separate events.", "current_reports": [ "R01" ], "existence": "Material information recorded in the preserved summary", "acquired_read": "Summary read; external materials not read", "observation": "The 2026-08-26 reference date, empty correction and deliberation values, Korean and English text, parent C54, original axes and subclaim D are preserved. Original pending and production-eligible markers are historical.", "condition_status": "Partly verified", "status_reason": "Only the relevant information from the stored summary is recorded. Detailed original materials have not been read, and the original content review remains incomplete. The absent subclaim score is not filled in. The original content review remains incomplete; model, content and scope review activation and completion remain false, added assessment units are 0 and the final independent count is unrecorded.", "missingness": "The absent subclaim score is not filled in. The original content review remains incomplete; model, content and scope review activation and completion remain false, added assessment units are 0 and the final independent count is unrecorded.", "uncertainty": "Unread/unverified does not mean zero effect, zero events, safety or new clinical certification.", "review_date": null, "reading_status": "Full text not read · Stored summary read", "additional_material": "The absent subclaim score is not filled in. The original content review remains incomplete; model, content and scope review activation and completion remain false, added assessment units are 0 and the final independent count is unrecorded." } ], "reports": [ { "report_id": "R01", "title": "Waziry R, Ryan CP, Corcoran DL, et al. Effect of long-term caloric restriction on DNA methylation measures of biological aging in healthy adults from the CALERIE trial. Nat Aging. 2023;3:248-257. PMID: 37118425.", "url": "https://pubmed.ncbi.nlm.nih.gov/37118425/", "report_type": "Post hoc DNA-analysis report from the parent randomized trial", "existence": "Materials preserved in original citation and evidence lists", "acquired_read": "Summary read; external materials not read", "study_family": "F01", "independent_replication": false, "observed_result": "Only DunedinPACE changed: d=−0.29 at 12 months and d=−0.25 at 24 months; the other two clocks were null", "result_level": "Stored evidence-list and narrative summaries; not direct reading of the original paper or registration", "source_evidence_date": "2026-08-26", "materials": [ { "material_type": "local_summary", "public_status": "Original source link preserved", "access_status": "Summary read", "acquired": true, "actually_read": "Summary read", "as_of": "2026-08-26", "qualification": "Preserved parent verdict summary" }, { "material_type": "abstract", "public_status": "Original source link preserved", "access_status": "Not read", "acquired": false, "actually_read": "Not read", "as_of": "2026-08-26", "qualification": "External material unread; a URL does not mean acquisition, reading or completed review" }, { "material_type": "fulltext", "public_status": "No separate URL in the original material", "access_status": "Not read", "acquired": false, "actually_read": "Not read", "as_of": "2026-08-26", "qualification": "External material unread; a URL does not mean acquisition, reading or completed review" }, { "material_type": "registration", "public_status": "No separate URL in the original material", "access_status": "Not read", "acquired": false, "actually_read": "Not read", "as_of": "2026-08-26", "qualification": "External material unread; a URL does not mean acquisition, reading or completed review" }, { "material_type": "protocol_sap", "public_status": "No separate URL in the original material", "access_status": "Not read", "acquired": false, "actually_read": "Not read", "as_of": "2026-08-26", "qualification": "External material unread; a URL does not mean acquisition, reading or completed review" } ], "links": [ { "label": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/37118425/" } ] } ], "method_sources": [ { "id": "M03", "version": "Cochrane Handbook 6.5 (2024), chapter updated 2023-08", "url": "https://www.cochrane.org/authors/handbooks-and-manuals/handbook/current/chapter-03", "section": "§3.1, §3.2.1–3.2.3, MECIR C5/C7/C13", "official_principle": "Clearly record the question, eligibility conditions and reasons for changes, and distinguish variations in interventions.", "review_level": "Preserved methodology connections were reused for preparation. No new review of methodological content, reassessment or external-link checks were performed." }, { "id": "M04", "version": "Cochrane Handbook 6.5.1 (2025), chapter updated 2025-03", "url": "https://www.cochrane.org/authors/handbooks-and-manuals/handbook/current/chapter-04", "section": "§4.5, §4.6.1–4.6.3, MECIR C42", "official_principle": "Distinguish studies from reports and link them; do not omit a study solely because results are unreported.", "review_level": "Preserved methodology connections were reused for preparation. No new review of methodological content, reassessment or external-link checks were performed." }, { "id": "M08", "version": "Cochrane Handbook 6.5 (2024), chapter updated 2019-10", "url": "https://www.cochrane.org/authors/handbooks-and-manuals/handbook/current/chapter-08", "section": "§8.1–8.2.3, Table 8.2.a", "official_principle": "Define the outcome and effect of interest, then examine randomization, deviations, missing data, measurement and selective reporting.", "review_level": "Preserved methodology connections were reused for preparation. No new review of methodological content, reassessment or external-link checks were performed." }, { "id": "M15", "version": "Cochrane Handbook 6.5 (2024), chapter updated 2023-08", "url": "https://www.cochrane.org/authors/handbooks-and-manuals/handbook/current/chapter-15", "section": "§15.2.4, §15.3.1–15.3.3", "official_principle": "Distinguish effect estimates, intervals, importance and applicability. Sample size alone does not determine precision.", "review_level": "Preserved methodology connections were reused for preparation. No new review of methodological content, reassessment or external-link checks were performed." }, { "id": "M19", "version": "Cochrane Handbook 6.5 (2024), chapter updated 2019-10", "url": "https://www.cochrane.org/authors/handbooks-and-manuals/handbook/current/chapter-19", "section": "§19.1.2, §19.2.1–19.2.4", "official_principle": "Harm questions may require different designs, populations and follow-up from efficacy questions. Case reports do not establish incidence rates.", "review_level": "Preserved methodology connections were reused for preparation. No new review of methodological content, reassessment or external-link checks were performed." } ], "status_dictionary": { "Partly verified": "Some information exists in the preserved review summary; detailed original materials remain unverified. This does not certify a passed review or clinical efficacy.", "Unverified": "Detailed materials for the selected condition could not be established within the stored scope; this does not mean zero effect, zero studies or safety", "Review incomplete": "Review of existing content incomplete", "Met": "Use only within the relevant conditions after existing content review; no automatic designation in this preparation", "Not reported": "Distinguish only within materials actually read; do not turn unread external materials into unreported findings", "Inaccessible": "Not applied during this preparation because actual access was not tested", "Not applicable": "Use with a reason for non-applicability based on an explicit question boundary", "Conflicting data": "Judge after actual material comparison; review was not performed during this preparation", "Insufficient evidence for the conditions": "Do not use as a clinical grade encompassing currently unread materials" }, "study_overlap": { "state": "Unverified", "unique_trial_count": null, "unique_participant_count": null, "reason": "Post hoc DNA analysis of the CALERIE parent randomized trial; the two subclaim sheets, measures and time points are shared, rather than separate new trials. Family IDs connect relationships and do not certify unique trial counts; reports, time points, measures, sibling sheets and the same participants are not newly summed as independent evidence." }, "history": [ { "date": "2026-10-11", "kind": "Separation of public-facing pages and materials", "view_version": "1.0", "change": "Public pages and materials were prepared from the existing 27 items. No new clinical search, verification-status change or scoring was performed." } ], "language": "en", "translation_date": "2026-10-11" }